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Int. J. Mol. Sci. 2014, 15(9), 16331-16350; doi:10.3390/ijms150916331

Ectopic Expression of CsCTR1, a Cucumber CTR-Like Gene, Attenuates Constitutive Ethylene Signaling in an Arabidopsis ctr1-1 Mutant and Expression Pattern Analysis of CsCTR1 in Cucumber (Cucumis sativus)

1
Plant Science Department, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China
2
National-Local Joint Engineering Research Center of Biodiagnostics & Biotherapy, Xi'an Jiaotong University, Xi'an 710004, Shaanxi, China
*
Author to whom correspondence should be addressed.
Received: 3 July 2014 / Revised: 13 August 2014 / Accepted: 25 August 2014 / Published: 15 September 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

The gaseous plant hormone ethylene regulates many aspects of plant growth, development and responses to the environment. Constitutive triple response 1 (CTR1) is a central regulator involved in the ethylene signal transduction pathway. To obtain a better understanding of this particular pathway in cucumber, the cDNA-encoding CTR1 (designated CsCTR1) was isolated from cucumber. A sequence alignment and phylogenetic analyses revealed that CsCTR1 has a high degree of homology with other plant CTR1 proteins. The ectopic expression of CsCTR1 in the Arabidopsis ctr1-1 mutant attenuates constitutive ethylene signaling of this mutant, suggesting that CsCTR1 indeed performs its function as negative regulator of the ethylene signaling pathway. CsCTR1 is constitutively expressed in all of the examined cucumber organs, including roots, stems, leaves, shoot apices, mature male and female flowers, as well as young fruits. CsCTR1 expression gradually declined during male flower development and increased during female flower development. Additionally, our results indicate that CsCTR1 can be induced in the roots, leaves and shoot apices by external ethylene. In conclusion, this study provides a basis for further studies on the role of CTR1 in the biological processes of cucumber and on the molecular mechanism of the cucumber ethylene signaling pathway. View Full-Text
Keywords: Cucumis sativus; constitutive triple response 1 (CTR1); ectopic expression; ethylene signaling; expression analysis Cucumis sativus; constitutive triple response 1 (CTR1); ectopic expression; ethylene signaling; expression analysis
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Bie, B.; Sun, J.; Pan, J.; He, H.; Cai, R. Ectopic Expression of CsCTR1, a Cucumber CTR-Like Gene, Attenuates Constitutive Ethylene Signaling in an Arabidopsis ctr1-1 Mutant and Expression Pattern Analysis of CsCTR1 in Cucumber (Cucumis sativus). Int. J. Mol. Sci. 2014, 15, 16331-16350.

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