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Int. J. Mol. Sci. 2014, 15(8), 14364-14371; doi:10.3390/ijms150814364

Cross-Species, Amplifiable Microsatellite Markers for Neoverrucid Barnacles from Deep-Sea Hydrothermal Vents Developed Using Next-Generation Sequencing

1
Marine Biophysics Unit, Okinawa Institute of Science and Technology Graduate University, 1919-1 Tancha, Onna, Okinawa 904-0495, Japan
2
Marine Genomics Unit, Okinawa Institute of Science and Technology Graduate University, 1919-1 Tancha, Onna, Okinawa 904-0495, Japan
3
Department of Marine Biodiversity Research, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan
4
Kochi Institute for Core Sample Research, Japan Agency for Marine-Earth Science and Technology, B-200 Monobe, Nankoku, Kochi 783-8502, Japan
*
Author to whom correspondence should be addressed.
Received: 19 June 2014 / Revised: 16 July 2014 / Accepted: 12 August 2014 / Published: 18 August 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Barnacles of the genus Neoverruca are abundant near deep-sea hydrothermal vents of the northwestern Pacific Ocean, and are useful for understanding processes of population formation and maintenance of deep-sea vent faunas. Using next-generation sequencing, we isolated 12 polymorphic microsatellite loci from Neoverruca sp., collected in the Okinawa Trough. These microsatellite loci revealed 2–19 alleles per locus. The expected and observed heterozygosities ranged from 0.286 to 1.000 and 0.349 to 0.935, respectively. Cross-species amplification showed that 9 of the 12 loci were successfully amplified for Neoverruca brachylepadoformis in the Mariana Trough. A pairwise FST value calculated using nine loci showed significant genetic differentiation between the two species. Consequently, the microsatellite markers we developed will be useful for further population genetic studies to elucidate genetic diversity, differentiation, classification, and evolutionary processes in the genus Neoverruca. View Full-Text
Keywords: deep-sea; microsatellites; Neoverruca; population genetics deep-sea; microsatellites; Neoverruca; population genetics
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Nakajima, Y.; Shinzato, C.; Khalturina, M.; Watanabe, H.; Inagaki, F.; Satoh, N.; Mitarai, S. Cross-Species, Amplifiable Microsatellite Markers for Neoverrucid Barnacles from Deep-Sea Hydrothermal Vents Developed Using Next-Generation Sequencing. Int. J. Mol. Sci. 2014, 15, 14364-14371.

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