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Int. J. Mol. Sci. 2014, 15(8), 14180-14190; doi:10.3390/ijms150814180

Effects of Pulsed Electromagnetic Field on Differentiation of HUES-17 Human Embryonic Stem Cell Line

1
Department of Radiation Medicine, the Fourth Military Medical University, Xi'an 710032, China
2
Experiment Teaching Center, the Fourth Military Medical University, Xi'an 710032, China
3
Department of Pathology and Pathophysiology, the Fourth Military Medical University, Xi'an 710032, China
4
Department of Neurosurgery, the First Hospital of Jilin University, Changchun 130021, China
5
Department of Pharmaceutics, the Fourth Military Medical University, Xi'an 710032, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 11 June 2014 / Revised: 14 July 2014 / Accepted: 25 July 2014 / Published: 14 August 2014
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
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Abstract

Electromagnetic fields are considered to potentially affect embryonic development, but the mechanism is still unknown. In this study, human embryonic stem cell (hESC) line HUES-17 was applied to explore the mechanism of exposure on embryonic development to pulsed electromagnetic field (PEMF) for 400 pulses at different electric field intensities and the differentiation of HUES-17 cells was observed after PEMF exposure. The expression of alkaline phosphatase (AP), stage-specific embryonic antigen-3 (SSEA-3), SSEA-4 and the mRNA level and protein level of Oct4, Sox2 and Nanog in HUES-17 cells remained unchanged after PEMF exposure at the electric field intensities of 50, 100, 200 or 400 kV/m. Four hundred pulses PEMF exposure at the electric field intensities of 50, 100, 200 or 400 kV/m did not affect the differentiation of HUES-17 cells. The reason why electromagnetic fields affect embryonic development may be due to other mechanisms rather than affecting the differentiation of embryonic stem cells. View Full-Text
Keywords: PEMF; hESC; differentiation PEMF; hESC; differentiation
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Wu, Y.-L.; Ma, S.-R.; Peng, T.; Teng, Z.-H.; Liang, X.-Y.; Guo, G.-Z.; Zhang, H.-F.; Li, K.-C. Effects of Pulsed Electromagnetic Field on Differentiation of HUES-17 Human Embryonic Stem Cell Line. Int. J. Mol. Sci. 2014, 15, 14180-14190.

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