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Int. J. Mol. Sci. 2014, 15(8), 14191-14219; doi:10.3390/ijms150814191

Complete Proteome of a Quinolone-Resistant Salmonella Typhimurium Phage Type DT104B Clinical Strain

1
Institute for Biotechnology and Bioengineering, Centre of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal
2
Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal
3
Centre of Studies of Animal and Veterinary Sciences, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal
4
Veterinary Science Department, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal
5
BIOSCOPE group, REQUIMTE-CQFB, Chemistry Department, Faculty of Science and Technology, University NOVA of Lisbon, 2829-516 Monte de Caparica, Portugal
6
Departamento de Biología Molecular (Universidad de Cantabria) and Instituto de Biomedicina y Biotecnología de Cantabria IBBTEC (UC-SODERCAN-CSIC), Santander 39011, Spain
7
Microbiología Molecular, Centro de Investigación Biomédica de La Rioja, C/Piqueras 98, 26006 Logroño, La Rioja, Spain
8
Bioquímica y Biología Molecular, Universidad de La Rioja, Av. Madre de Dios 51, 26006 Logroño, La Rioja, Spain
*
Author to whom correspondence should be addressed.
Received: 8 June 2014 / Revised: 27 June 2014 / Accepted: 25 July 2014 / Published: 15 August 2014
(This article belongs to the Collection Advances in Proteomic Research)
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Abstract

Salmonellosis is one of the most common and widely distributed foodborne diseases. The emergence of Salmonella strains that are resistant to a variety of antimicrobials is a serious global public health concern. Salmonella enterica serovar Typhimurium definitive phage type 104 (DT104) is one of these emerging epidemic multidrug resistant strains. Here we collate information from the diverse and comprehensive range of experiments on Salmonella proteomes that have been published. We then present a new study of the proteome of the quinolone-resistant Se20 strain (phage type DT104B), recovered after ciprofloxacin treatment and compared it to the proteome of reference strain SL1344. A total of 186 and 219 protein spots were recovered from Se20 and SL1344 protein extracts, respectively, after two-dimensional gel electrophoresis. The signatures of 94% of the protein spots were successfully identified through matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS). Three antimicrobial resistance related proteins, whose genes were previously detected by polymerase chain reaction (PCR), were identified in the clinical strain. The presence of these proteins, dihydropteroate synthase type-2 (sul2 gene), aminoglycoside resistance protein A (strA gene) and aminoglycoside 6'-N-acetyltransferase type Ib-cr4 (aac(6')-Ib-cr4 gene), was confirmed in the DT104B clinical strain. The aac(6')-Ib-cr4 gene is responsible for plasmid-mediated aminoglycoside and quinolone resistance. This is a preliminary analysis of the proteome of these two S. Typhimurium strains and further work is being developed to better understand how antimicrobial resistance is developing in this pathogen. View Full-Text
Keywords: Salmonella enterica serovar Typhimurium; DT104B; SL1344; proteome; aminoglycoside 6'-N-acetyltransferase type Ib-cr4 Salmonella enterica serovar Typhimurium; DT104B; SL1344; proteome; aminoglycoside 6'-N-acetyltransferase type Ib-cr4
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Correia, S.; Nunes-Miranda, J.D.; Pinto, L.; Santos, H.M.; de Toro, M.; Sáenz, Y.; Torres, C.; Capelo, J.L.; Poeta, P.; Igrejas, G. Complete Proteome of a Quinolone-Resistant Salmonella Typhimurium Phage Type DT104B Clinical Strain. Int. J. Mol. Sci. 2014, 15, 14191-14219.

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