Next Article in Journal
Effects of Pulsed Electromagnetic Field on Differentiation of HUES-17 Human Embryonic Stem Cell Line
Previous Article in Journal
Kinetics and Quantitative Structure—Activity Relationship Study on the Degradation Reaction from Perfluorooctanoic Acid to Trifluoroacetic Acid
Article Menu
Issue 8 (August) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2014, 15(8), 14166-14179; doi:10.3390/ijms150814166

Modulation of Nitrosative Stress via Glutathione-Dependent Formaldehyde Dehydrogenase and S-Nitrosoglutathione Reductase

1
Department of Bioscience and Biotechnology and Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung 202, Taiwan
2
Institute of Biotechnology, National Changhua University of Education, Changhua 500, Taiwan
3
Department of Chemistry, Western Illinois University, Macomb, IL 61455, USA
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 17 June 2014 / Revised: 28 July 2014 / Accepted: 8 August 2014 / Published: 14 August 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
View Full-Text   |   Download PDF [3292 KB, uploaded 14 May 2015]   |  

Abstract

Glutathione-dependent formaldehyde dehydrogenase (GFD) from Taiwanofungus camphorata plays important roles in formaldehyde detoxification and antioxidation. The enzyme is bifunctional. In addition to the GFD activity, it also functions as an effective S-nitrosoglutathione reductase (GSNOR) against nitrosative stress. We investigated the modulation of HEK (human embryonic kidney) 293T cells under nitrosative stress by transfecting a codon optimized GFD cDNA from Taiwanofungus camphorata (Tc-GFD-O) to these cells. The parental and transfected HEK 293T cells were then subjected to S-nitrosoglutathione treatment to induce nitrosative stress. The results showed that in Tc-GFD-O-transfected 293T cells, the expression and activity of GFD increased. Additionally, these cells under the nitrosative stress induced by S-nitrosoglutathione showed both higher viability and less apoptosis than the parental 293T cells. This finding suggests that the Tc-GFD-O in HEK 293T cells may provide a protective function under nitrosative stress. View Full-Text
Keywords: Taiwanofungus camphorata (formerly named Antrodia camphorata); glutathione-dependent formaldehyde dehydrogenase (GFD); S-nitrosoglutathione (GSNO); S-nitrosoglutathione reductase (GSNOR) Taiwanofungus camphorata (formerly named Antrodia camphorata); glutathione-dependent formaldehyde dehydrogenase (GFD); S-nitrosoglutathione (GSNO); S-nitrosoglutathione reductase (GSNOR)
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Ken, C.-F.; Huang, C.-Y.; Wen, L.; Huang, J.-K.; Lin, C.-T. Modulation of Nitrosative Stress via Glutathione-Dependent Formaldehyde Dehydrogenase and S-Nitrosoglutathione Reductase. Int. J. Mol. Sci. 2014, 15, 14166-14179.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top