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Int. J. Mol. Sci. 2014, 15(8), 13406-13423; doi:10.3390/ijms150813406

Effects of Lead on the Morphology and Structure of the Nucleolus in the Root Tip Meristematic Cells of Allium cepa L.

1
Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin 300387, China
2
Institute of Genetics and Physiological, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China
3
School of Life Science, South China Normal University, Guangzhou 510631, China
*
Author to whom correspondence should be addressed.
Received: 20 June 2014 / Revised: 15 July 2014 / Accepted: 24 July 2014 / Published: 31 July 2014
(This article belongs to the Section Molecular Toxicology)
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Abstract

To study the toxic mechanisms of lead (Pb) in plants, the effects of Pb on the morphology and structure of the nucleolus in root tip meristematic cells of Allium cepa var. agrogarum L. were investigated. Fluorescence labeling, silver-stained indirect immunofluorescent microscopy and western blotting were used. Fluorescence labeling showed that Pb ions were localized in the meristematic cells and the uptake and accumulation of Pb increased with treatment time. At low concentrations of Pb (1–10 μM) there were persistent nucleoli in some cells during mitosis, and at high concentration (100 μM) many of the nucleolar organizing regions were localized on sticky chromosomes in metaphase and anaphase cells. Pb induced the release of particles containing argyrophilic proteins to be released from the nucleus into the cytoplasm. These proteins contained nucleophosmin and nucleolin. Pb also caused the extrusion of fibrillarin from the nucleus into the cytoplasm. Western blotting demonstrated the increased expression of these three major nucleolar proteins under Pb stress. View Full-Text
Keywords: Allium cepa var. agrogarum L.; lead (Pb); nucleolus; nucleophosmin; nucleolin; fibrillarin Allium cepa var. agrogarum L.; lead (Pb); nucleolus; nucleophosmin; nucleolin; fibrillarin
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Jiang, Z.; Zhang, H.; Qin, R.; Zou, J.; Wang, J.; Shi, Q.; Jiang, W.; Liu, D. Effects of Lead on the Morphology and Structure of the Nucleolus in the Root Tip Meristematic Cells of Allium cepa L.. Int. J. Mol. Sci. 2014, 15, 13406-13423.

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