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Int. J. Mol. Sci. 2014, 15(7), 11817-11831; https://doi.org/10.3390/ijms150711817

Inhibition of TRPM7 Channels Reduces Degranulation and Release of Cytokines in Rat Bone Marrow-Derived Mast Cells

1
Department of Respiratory Medicine, Sun Yat-sen Memorial Hospital, Institute for Respiratory Disease of Sun Yat-sen University, Sun Yat-sen University, Guangzhou 510120, China
2
Clinical Department of School of Medicine, Shenzhen University, Shenzhen 518060, China
3
Department of Respiratory Medicine, Mei Zhou People's Hospital, Meizhou Affiliated Hospital of Sun Yat-sen University, Meizhou 514031, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 18 May 2014 / Revised: 9 June 2014 / Accepted: 19 June 2014 / Published: 3 July 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Background: mast cells play an important role in airway inflammation in asthma. The transient receptor potential melastatin-like 7 (TRPM7) channel is expressed in primary human lung mast cells and plays a critical role for cell survival. This study aimed to investigate the role of TRPM7 on degranulation and release of cytokines in rat bone marrow-derived mast cells (BMMCs). Methods: the expression levels of TRPM7 were observed by immunocytochemistry and RT-PCR between normal and asthmatic rat BMMCs. TRPM7-specific shRNA and 2-aminoethoxydiphenyl borate (2-APB) and specific shTRPM7 were used to inhibit the function of TRPM7. Degranulation levels were analyzed by beta-hexosaminidase assay. Histamine, TNF-α, IL-6 and IL-13 levels were measured by ELISA. Results: the expression of TRPM7 was significantly higher in asthmatic rat BMMCs than in the normal control group. After application of 2-APB and down-regulation of TRPM7, the beta-hexosaminidase activity and secretion of histamine, IL-6, IL-13 and TNF-α were significantly decreased in the asthmatic group compared to the control group. Conclusion: this study indicates that TRPM7 channels may be involved in the process of degranulation and release of cytokines in rat bone marrow-derived mast cells. View Full-Text
Keywords: rat; asthma; bone marrow-derived mast cells (BMMCs); transient receptor potential melastatin 7 (TRPM7); 2-aminoethoxydiphenyl borate (2-APB); shRNA; cytokines rat; asthma; bone marrow-derived mast cells (BMMCs); transient receptor potential melastatin 7 (TRPM7); 2-aminoethoxydiphenyl borate (2-APB); shRNA; cytokines
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Huang, L.; Ng, N.-M.; Chen, M.; Lin, X.; Tang, T.; Cheng, H.; Yang, C.; Jiang, S. Inhibition of TRPM7 Channels Reduces Degranulation and Release of Cytokines in Rat Bone Marrow-Derived Mast Cells. Int. J. Mol. Sci. 2014, 15, 11817-11831.

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