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Int. J. Mol. Sci. 2014, 15(6), 9963-9978; doi:10.3390/ijms15069963
Article

Characterization of Two Homogalacturonan Pectins with Immunomodulatory Activity from Green Tea

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Received: 5 April 2014; in revised form: 25 April 2014 / Accepted: 20 May 2014 / Published: 4 June 2014
(This article belongs to the Section Bioactives and Nutraceuticals)
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Abstract: Two natural homogalacturonan (HG) pectins (MW ca. 20 kDa) were isolated from green tea based on their immunomodulatory activity. The crude tea polysaccharides (TPS1 and TPS2) were obtained from green tea leaves by hot water extraction and followed by 40% and 70% ethanol precipitation, respectively. Two homogenous water soluble polysaccharides (TPS1-2a and TPS1-2b) were obtained from TPS1 after purification with gel permeation, which gave a higher phagocytic effect than TPS2. A combination of composition, methylation and configuration analyses, as well as NMR (nuclear magnetic resonance) spectroscopy revealed that TPS1-2a and TPS1-2b were homogalacturonan (HG) pectins consisting of a backbone of 1,4-linked α-d-galacturonic acid (GalA) residues with 28.4% and 26.1% of carboxyl groups as methyl ester, respectively. The immunological assay results demonstrated that TPS1-2, which consisted mainly of HG pectins, showed phagocytosis-enhancing activity in HL-60 cells.
Keywords: Camellia sinensis; green tea; homogalacturonan; pectin; phagocytosis; immunomodulatory Camellia sinensis; green tea; homogalacturonan; pectin; phagocytosis; immunomodulatory
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Wang, H.; Wei, G.; Liu, F.; Banerjee, G.; Joshi, M.; Bligh, S.W.A.; Shi, S.; Lian, H.; Fan, H.; Gu, X.; Wang, S. Characterization of Two Homogalacturonan Pectins with Immunomodulatory Activity from Green Tea. Int. J. Mol. Sci. 2014, 15, 9963-9978.

AMA Style

Wang H, Wei G, Liu F, Banerjee G, Joshi M, Bligh SWA, Shi S, Lian H, Fan H, Gu X, Wang S. Characterization of Two Homogalacturonan Pectins with Immunomodulatory Activity from Green Tea. International Journal of Molecular Sciences. 2014; 15(6):9963-9978.

Chicago/Turabian Style

Wang, Huijun; Wei, Guodong; Liu, Fei; Banerjee, Gautam; Joshi, Manoj; Bligh, S. W.A.; Shi, Songshan; Lian, Hui; Fan, Hongwei; Gu, Xuelan; Wang, Shunchun. 2014. "Characterization of Two Homogalacturonan Pectins with Immunomodulatory Activity from Green Tea." Int. J. Mol. Sci. 15, no. 6: 9963-9978.



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