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Int. J. Mol. Sci. 2014, 15(6), 10999-11012; doi:10.3390/ijms150610999

The Effects of CoCl2 on HIF-1α Protein under Experimental Conditions of Autoprogressive Hypoxia Using Mouse Models

6,*  and 4,6,*
1 Department of Neurology, Affiliated Hospital of Tai Shan Medical University, Taishan 271000, China 2 Department of Intensive Care Unit , 2nd Affiliated Hospital of Baotou Medical College, Baotou 014030, China 3 Department of Medicine, University of Arizona, Tucson, AZ 85721, USA 4 Biomedicine Research Center and Basic Medical College, Baotou Medical College, Baotou 014060, China 5 Department of Anatomy, University of California, San Francisco, CA 94143, USA 6 Institute for Hypoxia Medicine, Xuanwu Hospital of Capital Medical University, Beijing 10054, China These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 28 February 2014 / Revised: 10 June 2014 / Accepted: 11 June 2014 / Published: 18 June 2014
(This article belongs to the Section Molecular Pathology)
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It is well known that cobalt chloride (CoCl2) can enhance the stability of hypoxia-inducible factor (HIF)-1α. The aim of this study is to detect the effect of CoCl2 on the hypoxia tolerance of mice which were repeatedly exposed to autoprogressive hypoxia. Balb/c mice were randomly divided into groups of chemical pretreatment and normal saline (NS), respectively injected with CoCl2 and NS 3 h before exposure to hypoxia for 0 run (H0), 1 run (H1), and 4 runs (H4). Western Blot, electrophoretic mobility shift assay (EMSA), extracellular recordings population spikes in area cornus ammonis I (CA 1) of mouse hippocampal slices and real-time were used in this study. Our results demonstrated that the tolerance of mice to hypoxia, the changes of HIF-1α protein level and HIF-1 DNA binding activity in mice hippocampus, the mRNA level of erythropoietin (EPO) and vascular endothelial growth factor (VEGF), and the disappearance time of population spikes of hippocampal slices were substantially different between the control group and the CoCl2 group. Over-induction of HIF-1α by pretreatment with CoCl2 before hypoxia did not increase the hypoxia tolerance.
Keywords: hypoxia preconditioning; hypoxia-inducible factor-1; hippocampus; CoCl2 hypoxia preconditioning; hypoxia-inducible factor-1; hippocampus; CoCl2
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Zhang, Y.-B.; Wang, X.; Meister, E.A.; Gong, K.-R.; Yan, S.-C.; Lu, G.-W.; Ji, X.-M.; Shao, G. The Effects of CoCl2 on HIF-1α Protein under Experimental Conditions of Autoprogressive Hypoxia Using Mouse Models. Int. J. Mol. Sci. 2014, 15, 10999-11012.

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