Abstract: Diarrhea and edematous disease are two major causes of mortality in postweaning piglets, and these conditions lead to huge economic losses in the swine industry. E. coli F18 is the primary causative agent of these two diseases. Bactericidal/permeability-increasing protein (BPI) plays an important role in the natural defense of the host. The aim of this study was to determine the correlation between BPI gene upstream CpG island methylation and mRNA expression. In this study, bisulfite sequencing PCR (BSP) was used to detect the methylation status of the BPI gene upstream CpG island and fluorescence quantitative PCR was used to detect BPI expression in the duodenum of piglets from birth to weaning age. BPI upstream CpG islands were shown to have many putative transcription factor binding sites, 10 CpG sites and every CpG site was methylated. The CpG island methylation level was lowest in 30-day piglets and was significantly lower than levels in 8-day piglets (p < 0.05). BPI mRNA expression was significantly higher in 30-day piglets than at any other age (p < 0.05). Pearson’s correlation analysis showed that the methylation status of the CpG island was negatively correlated with BPI mRNA expression. Statistical significances were found in CpG_1, CpG_3, CpG_4, CpG_7 and CpG_10 (p < 0.05). The data indicate that BPI expression is improved by demethylation of the BPI gene upstream CpG island. Furthermore, CpG_1, CpG_3, CpG_4, CpG_7 and CpG_10 may be critical sites in the regulation of BPI gene expression.
Keywords: pig; BPI gene; CpG island; methylation; BSP (bisulfite sequencing PCR)
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Wang, J.; Yin, X.; Sun, L.; Sun, S.; Zi, C.; Zhu, G.; Wu, S.; Bao, W. Correlation between BPI Gene Upstream CpG Island Methylation and mRNA Expression in Piglets. Int. J. Mol. Sci. 2014, 15, 10989-10998.
Wang J, Yin X, Sun L, Sun S, Zi C, Zhu G, Wu S, Bao W. Correlation between BPI Gene Upstream CpG Island Methylation and mRNA Expression in Piglets. International Journal of Molecular Sciences. 2014; 15(6):10989-10998.
Wang, Jing; Yin, Xuemei; Sun, Li; Sun, Shouyong; Zi, Chen; Zhu, Guoqiang; Wu, Shenglong; Bao, Wenbin. 2014. "Correlation between BPI Gene Upstream CpG Island Methylation and mRNA Expression in Piglets." Int. J. Mol. Sci. 15, no. 6: 10989-10998.