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Int. J. Mol. Sci. 2014, 15(5), 9103-9116; doi:10.3390/ijms15059103

Chemical Characterization and Antitumor Activities of Polysaccharide Extracted from Ganoderma lucidum

1
College of Horticulture and Landscape, Hunan Agricultural University, Changsha 410128, China
2
College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China
3
State Key Laboratory of Sub-Health Intervention Technology, State Administration of Traditional Chinese Medicine, Changsha 410128, China
*
Authors to whom correspondence should be addressed.
Received: 19 March 2014 / Revised: 1 May 2014 / Accepted: 4 May 2014 / Published: 22 May 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Ganoderma lucidum polysaccharide (GLP) is a biologically active substance reported to possess anti-tumor ability. Nonetheless, the mechanisms of GLP-stimulated apoptosis are still unclear. This study aims to determine the inhibitory and apoptosis-inducing effects of GLP on HCT-116 cells. We found that GLP reduced cell viability on HCT-116 cells in a time- and dose-dependent manner, which in turn, induced cell apoptosis. The observed apoptosis was characterized by morphological changes, DNA fragmentation, mitochondrial membrane potential decrease, S phase population increase, and caspase-3 and -9 activation. Furthermore, inhibition of c-Jun N-terminal kinase (JNK) by SP600125 led to a dramatic decrease of the GLP-induced apoptosis. Western blot analysis unveiled that GLP up-regulated the expression of Bax/Bcl-2, caspase-3 and poly (ADP-ribose) polymerase (PARP). These results demonstrate that apoptosis stimulated by GLP in human colorectal cancer cells is associated with activation of mitochondrial and mitogen-activated protein kinase (MAPK) pathways. View Full-Text
Keywords: human colon cancer cells; apoptosis; mitochondria; caspase; MAPK human colon cancer cells; apoptosis; mitochondria; caspase; MAPK
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Liang, Z.; Yi, Y.; Guo, Y.; Wang, R.; Hu, Q.; Xiong, X. Chemical Characterization and Antitumor Activities of Polysaccharide Extracted from Ganoderma lucidum. Int. J. Mol. Sci. 2014, 15, 9103-9116.

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