Tagging SNPs in the MTHFR Gene and Risk of Ischemic Stroke in a Chinese Population
Abstract
:1. Introduction
2. Results
3. Discussion
4. Material and Methods
4.1. Study Participants
4.2. SNP Selection
4.3. Laboratory Tests
4.4. Statistical Analysis
5. Conclusions
Conflicts of Interest
- Author ContributionsYi-Pin Zhou contributes to study conception and design; Bao-Sheng Zhou and Guo-Yun Bu contribute to data collection, biological experiments and drafting the article; Mu Li and Bin-Ge Chang contribute to biological experiments and data analysis.
References
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Characteristics | Cases (n = 543) | Controls (n = 655) | p value |
---|---|---|---|
Age (year) | 66 (61–70) | 66 (60–70) | 0.76 |
Sex (Male/Female) | 346/197 | 402/253 | 0.42 |
Smoking (Yes/No) | 224/319 | 215/440 | 0.003 |
Diabetes (Yes/No) | 137/406 | 81/574 | <0.001 |
Hypertension (Yes/No) | 293/250 | 222/433 | <0.001 |
BMI (kg/m2) | 24.3 (22.9–25.8) | 24.3 (23.0–25.7) | 0.55 |
Total cholesterol (mmol/L) | 4.70 (4.09–5.48) | 4.14 (3.60–5.04) | 0.82 |
HDL-C (mmol/L) | 1.20 (1.09–1.51) | 1.24 (1.08–1.56) | 0.008 |
LDL-C (mmol/L) | 2.82 (2.45–3.29) | 2.48 (2.16–3.03) | <0.001 |
Heart rate (bmp) | 72 (67–76) | 74 (68–78) | <0.001 |
SNP | Genotypes | Cases, n (%) | Controls, n (%) | OR (95% CI) † | OR (95% CI) ‡ | P trend |
---|---|---|---|---|---|---|
rs12121543 | CC | 362 (66.8) | 449 (68.7) | 1.00 | 1.00 | |
CA | 159 (29.3) | 185 (28.3) | 1.04 (0.80–1.36) | 0.99 (0.75–1.30) | ||
AA | 21 (3.9) | 20 (3.1) | 1.42 (0.75–2.71) | 1.45 (0.74–2.84) | 0.61 | |
CA + AA | 180 (33.2) | 205 (31.4) | 1.08 (0.84–1.39) | 1.03 (0.79–1.34) | ||
rs13306561 | TT | 432 (79.7) | 529 (80.9) | 1.00 | 1.00 | |
TC | 106 (19.6) | 121 (18.5) | 1.03 (0.76–1.39) | 0.95 (0.69–1.29) | ||
CC | 4 (0.7) | 4 (0.6) | 1.27 (0.30–5.37) | 1.36 (0.30–6.18) | 0.67 | |
TC + CC | 110 (20.3) | 125 (19.1) | 1.03 (0.77–1.39) | 0.96 (0.70–1.30) | ||
rs13306553 | TT | 435 (80.3) | 534 (81.7) | 1.00 | 1.00 | |
TC | 105 (19.3) | 116 (17.6) | 1.06 (0.78–1.44) | 0.97 (0.70–1.33) | ||
CC | 2 (0.4) | 4 (0.6) | 0.62 (0.11–3.60) | 0.71 (0.11–4.55) | 0.54 | |
TC + CC | 107 (19.7) | 120 (18.3) | 1.05 (0.78–1.41) | 0.96 (0.70–1.31) | ||
rs9651118 | TT | 230 (42.4) | 273 (41.7) | 1.00 | 1.00 | |
TC | 233 (43.0) | 291 (44.5) | 0.93 (0.72–1.20) | 0.93 (0.71–1.21) | ||
CC | 79 (14.6) | 90 (13.8) | 1.05 (0.73–1.51) | 1.00 (0.69–1.46) | 0.66 | |
TC + CC | 312 (57.6) | 381 (58.3) | 0.96 (0.75–1.21) | 0.95 (0.74–1.21) | ||
rs1801133 | CC | 160 (29.5) | 242 (37.0) | 1.00 | 1.00 | |
CT | 270 (49.8) | 308 (47.1) | 1.34 (1.03–1.76) | 1.31 (0.99–1.73) | ||
TT | 112 (20.7) | 104 (15.9) | 1.64 (1.16–2.31) | 1.82 (1.27–2.61) | 0.04 | |
CT + TT | 382 (70.5) | 412 (63.0) | 1.42 (1.10–1.83) | 1.44 (1.10–1.87) | ||
rs2274976 | GG | 433 (79.9) | 542 (82.9) | 1.00 | 1.00 | |
GA | 104 (19.2) | 107 (16.4) | 1.17 (0.86–1.60) | 1.10 (0.80–1.52) | ||
AA | 5 (0.9) | 5 (0.8) | 1.34 (0.37–4.82) | 1.50 (0.39–5.69) | 0.21 | |
GA + AA | 109 (20.1) | 112 (17.1) | 1.18 (0.87–1.60) | 1.12 (0.81–1.53) | ||
rs4846048 | AA | 424 (78.5) | 538 (82.3) | 1.00 | 1.00 | |
AG | 108 (20.0) | 111 (17.0) | 1.23 (0.90–1.66) | 1.19 (0.87–1.64) | ||
GG | 8 (1.5) | 5 (0.8) | 2.60 (0.81–8.38) | 2.82 (0.83–9.54) | 0.12 | |
AG + GG | 116 (21.5) | 116 (17.7) | 1.28 (0.95–1.72) | 1.25 (0.92–1.70) | ||
rs1801131 | AA | 333 (61.4) | 448 (68.5) | 1.00 | 1.00 | |
AC | 174 (32.1) | 182 (27.8) | 1.27 (0.97–1.64) | 1.19 (0.91–1.57) | ||
CC | 35 (6.5) | 24 (3.7) | 1.97 (1.13–3.45) | 1.99 (1.12–3.56) | 0.03 | |
AC + CC | 209 (38.6) | 206 (31.5) | 1.35 (1.05–1.73) | 1.29 (0.99–1.66) | ||
rs17037396 | CC | 418 (77.1) | 500 (76.3) | 1.00 | 1.00 | |
CT | 112 (20.7) | 142 (21.7) | 0.95 (0.71–1.27) | 0.96 (0.71–1.30) | ||
TT | 12 (2.2) | 13 (2.0) | 1.22 (0.54–2.79) | 1.13 (0.49–2.62) | 0.71 | |
CT + TT | 124 (22.9) | 155 (23.7) | 0.97 (0.73–1.29) | 0.97 (0.73–1.31) |
Haplotype * | Cases, % | Controls, % | OR (95% CI) † | OR (95% CI) ‡ |
---|---|---|---|---|
CTTTGA | 38.7 | 38.7 | 1.00 | 1.00 |
CTCCGA | 33.6 | 35.7 | 0.96 (0.79–1.17) | 0.92 (0.76–1.12) |
ATTCGC | 6.8 | 8.7 | 0.79 (0.56–1.12) | 0.76 (0.54–1.08) |
ACTCAC | 7.5 | 7.7 | 0.94 (0.67–1.33) | 0.88 (0.62–1.24) |
CTTCGA | 3.2 | 6.6 | 0.55 (0.36–0.84) | 0.53 (0.35–0.82) |
SNP | M/m | tHcy (mmol/L) | p | ||
---|---|---|---|---|---|
MM | Mm | mm | |||
rs12121543 | C/A | 12.4 (10.1–14.6) | 12.1 (9.7–13.7) | 10.8 (8.5–13.4) | 0.26 |
rs13306561 | T/C | 12.1 (10.0–14.4) | 12.3 (10.2–14.1) | 9.1 (8.1–11.8) | 0.45 |
rs13306553 | T/C | 12.1 (10.0–14.4) | 12.4 (10.1–14.2) | 9.1 (8.0–11.8) | 0.45 |
rs9651118 | T/C | 12.5 (9.8–14.9) | 12.0 (10.0–13.8) | 11.5 (9.5–14.1) | 0.02 |
rs1801133 | C/T | 10.9 (9.2–12.8) | 12.8 (10.9–14.6) | 13.1 (10.0–16.8) | <0.0001 |
rs2274976 | G/A | 12.1 (10.0–14.4) | 12.3 (10.0–13.9) | 9.8 (8.4–13.7) | 0.57 |
rs4846048 | A/G | 12.3 (10.1–14.4) | 11.6 (9.4–13.6) | 11.1 (10.3–11.6) | 0.36 |
rs1801131 | A/C | 12.4 (10.0–14.6) | 12.2 (10.0–13.8) | 10.4 (8.6–13.4) | 0.36 |
rs17037396 | C/T | 12.2 (10.0–14.4) | 11.9 (9.9–14.4) | 11.7 (11.4–12.9) | 0.94 |
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Zhou, B.-S.; Bu, G.-Y.; Li, M.; Chang, B.-G.; Zhou, Y.-P. Tagging SNPs in the MTHFR Gene and Risk of Ischemic Stroke in a Chinese Population. Int. J. Mol. Sci. 2014, 15, 8931-8940. https://doi.org/10.3390/ijms15058931
Zhou B-S, Bu G-Y, Li M, Chang B-G, Zhou Y-P. Tagging SNPs in the MTHFR Gene and Risk of Ischemic Stroke in a Chinese Population. International Journal of Molecular Sciences. 2014; 15(5):8931-8940. https://doi.org/10.3390/ijms15058931
Chicago/Turabian StyleZhou, Bao-Sheng, Guo-Yun Bu, Mu Li, Bin-Ge Chang, and Yi-Pin Zhou. 2014. "Tagging SNPs in the MTHFR Gene and Risk of Ischemic Stroke in a Chinese Population" International Journal of Molecular Sciences 15, no. 5: 8931-8940. https://doi.org/10.3390/ijms15058931