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Int. J. Mol. Sci. 2014, 15(5), 8526-8538; doi:10.3390/ijms15058526

MicroRNA-199a-5p Affects Porcine Preadipocyte Proliferation and Differentiation

1
Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China
2
Vitamin D Research Institute, Shaanxi University of Technology, Hanzhong 723000, China
*
Author to whom correspondence should be addressed.
Received: 12 March 2014 / Revised: 31 March 2014 / Accepted: 4 May 2014 / Published: 14 May 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

MicroRNAs (miRNAs), a class of small non-coding RNAs, have emerged as novel and potent regulators of adipogenesis. However, few miRNAs have been fully investigated in porcine adipogenesis, given the fact that pig is not only an apropos model of human obesity research, but also a staple meat source of human diet. In this study, we showed that miRNA-199a-5p is highly expressed in porcine subcutaneous fat deposits compared to several other tissue types and organs measured alongside. Overexpression of miR-199a-5p in porcine preadipocytes significantly promoted cell proliferation while attenuating the lipid deposition in porcine adipocytes. By target gene prediction and experimental validation, we demonstrated that caveolin-1 (Cav-1) may be a bona fide target of miR-199a-5p in porcine adipocytes, accounting for some of miR-199a-5p’s functions. Taken together, our data established a role of miR-199a-5p in porcine preadipocyte proliferation and differentiation, which is at least partially played by downregulating Cav-1. View Full-Text
Keywords: porcine preadipocyte; miR-199a-5p; proliferation; differentiation porcine preadipocyte; miR-199a-5p; proliferation; differentiation
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Shi, X.-E.; Li, Y.-F.; Jia, L.; Ji, H.-L.; Song, Z.-Y.; Cheng, J.; Wu, G.-F.; Song, C.-C.; Zhang, Q.-L.; Zhu, J.-Y.; Yang, G.-S. MicroRNA-199a-5p Affects Porcine Preadipocyte Proliferation and Differentiation. Int. J. Mol. Sci. 2014, 15, 8526-8538.

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