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Int. J. Mol. Sci. 2014, 15(5), 8473-8490; doi:10.3390/ijms15058473

Arabidopsis ABA Receptor RCAR1/PYL9 Interacts with an R2R3-Type MYB Transcription Factor, AtMYB44

1
Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China
2
State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu 610065, China
3
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences/Key Laboratory of Crop Biotechnology, Ministry of Agriculture, Beijing 100081, China
*
Author to whom correspondence should be addressed.
Received: 22 March 2014 / Revised: 5 May 2014 / Accepted: 5 May 2014 / Published: 13 May 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Abscisic acid (ABA) signaling plays important roles in plant growth, development and adaptation to various stresses. RCAR1/PYL9 has been known as a cytoplasm and nuclear ABA receptor in Arabidopsis. To obtain further insight into the regulatory mechanism of RCAR1/PYL9, a yeast two-hybrid approach was performed to screen for RCAR1/PYL9-interacting proteins and an R2R3-type MYB transcription factor, AtMYB44, was identified. The interaction between RCAR1/PYL9 and AtMYB44 was further confirmed by glutathione S-transferase (GST) pull-down and bimolecular fluorescence complementation (BiFC) assays. Gene expression analysis showed that AtMYB44 negatively regulated the expression of ABA-responsive gene RAB18, in contrast to the opposite role reported for RCAR1/PYL9. Competitive GST pull-down assay and analysis of phosphatase activity demonstrated that AtMYB44 and ABI1 competed for binding to RCAR1/PYL9 and thereby reduced the inhibitory effect of RCAR1/PYL9 on ABI1 phosphatase activity in the presence of ABA in vitro. Furthermore, transient activation assay in protoplasts revealed AtMYB44 probably also decreased RCAR1/PYL9-mediated inhibition of ABI1 activity in vivo. Taken together, our work provides a reasonable molecular mechanism of AtMYB44 in ABA signaling. View Full-Text
Keywords: ABA; signaling; RCAR1/PYL9; receptor; AtMYB44; ABI1 ABA; signaling; RCAR1/PYL9; receptor; AtMYB44; ABI1
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Li, D.; Li, Y.; Zhang, L.; Wang, X.; Zhao, Z.; Tao, Z.; Wang, J.; Wang, J.; Lin, M.; Li, X.; Yang, Y. Arabidopsis ABA Receptor RCAR1/PYL9 Interacts with an R2R3-Type MYB Transcription Factor, AtMYB44. Int. J. Mol. Sci. 2014, 15, 8473-8490.

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