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Int. J. Mol. Sci. 2014, 15(5), 8316-8334; doi:10.3390/ijms15058316

Silencing of the CaCP Gene Delays Salt- and Osmotic-Induced Leaf Senescence in Capsicum annuum L.

1
College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, China
2
State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling 712100, Shaanxi, China
3
Institute of Vegetables, Hangzhou Academy of Agricultural Sciences, Hangzhou 311104, Zhejiang, China
*
Author to whom correspondence should be addressed.
Received: 28 March 2014 / Revised: 23 April 2014 / Accepted: 28 April 2014 / Published: 12 May 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Cysteine proteinases have been known to participate in developmental processes and in response to stress in plants. Our present research reported that a novel CP gene, CaCP, was involved in leaf senescence in pepper (Capsicum annuum L.). The full-length CaCP cDNA is comprised of 1316 bp, contains 1044 nucleotides in open reading frame (ORF), and encodes a 347 amino acid protein. The deduced protein belongs to the papain-like cysteine proteases (CPs) superfamily, containing a highly conserved ERFNIN motif, a GCNGG motif and a conserved catalytic triad. This protein localized to the vacuole of plant cells. Real-time quantitative PCR analysis revealed that the expression level of CaCP gene was dramatically higher in leaves and flowers than that in roots, stems and fruits. Moreover, CaCP transcripts were induced upon during leaf senescence. CaCP expression was upregulated by plant hormones, especially salicylic acid. CaCP was also significantly induced by abiotic and biotic stress treatments, including high salinity, mannitol and Phytophthora capsici. Loss of function of CaCP using the virus-induced gene-silencing technique in pepper plants led to enhanced tolerance to salt- and osmotic-induced stress. Taken together, these results suggest that CaCP is a senescence-associated gene, which is involved in developmental senescence and regulates salt- and osmotic-induced leaf senescence in pepper. View Full-Text
Keywords: pepper; cysteine proteinase; leaf senescence; osmotic stresses pepper; cysteine proteinase; leaf senescence; osmotic stresses
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Xiao, H.-J.; Yin, Y.-X.; Chai, W.-G.; Gong, Z.-H. Silencing of the CaCP Gene Delays Salt- and Osmotic-Induced Leaf Senescence in Capsicum annuum L.. Int. J. Mol. Sci. 2014, 15, 8316-8334.

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