Next Article in Journal
Effect of Black Soybean Koji Extract on Glucose Utilization and Adipocyte Differentiation in 3T3-L1 Cells
Next Article in Special Issue
Access of Hydrogen-Radicals to the Peptide-Backbone as a Measure for Estimating the Flexibility of Proteins Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry
Previous Article in Journal
Betulinic Acid in Complex with a Gamma-Cyclodextrin Derivative Decreases Proliferation and in Vivo Tumor Development of Non-Metastatic and Metastatic B164A5 Cells
Previous Article in Special Issue
Recent Advances in Bacteria Identification by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Using Nanomaterials as Affinity Probes
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2014, 15(5), 8256-8279; doi:10.3390/ijms15058256

Legionella dumoffii Utilizes Exogenous Choline for Phosphatidylcholine Synthesis

1
Department of Genetics and Microbiology, Institute of Microbiology and Biotechnology, Maria Curie-Sklodowska University, Akademicka 19 St., 20-033 Lublin, Poland
2
Department of Virology and Immunology, Institute of Microbiology and Biotechnology, Maria Curie-Sklodowska University, Akademicka 19 St., 20-033 Lublin, Poland
3
Mass Spectrometry Group, Institute of Organic Chemistry Polish Academy of Sciences, Kasprzaka 44/52 St., 01-224 Warsaw, Poland
*
Author to whom correspondence should be addressed.
Received: 12 March 2014 / Revised: 20 April 2014 / Accepted: 23 April 2014 / Published: 9 May 2014
(This article belongs to the Special Issue Mass Spectrometry Application in Biology)
View Full-Text   |   Download PDF [915 KB, uploaded 19 June 2014]   |  

Abstract

Phosphatidycholine (PC) is the major membrane-forming phospholipid in eukaryotes but it has been found in only a limited number of prokaryotes. Bacteria synthesize PC via the phospholipid N-methylation pathway (Pmt) or via the phosphatidylcholine synthase pathway (Pcs) or both. Here, we demonstrated that Legionella dumoffii has the ability to utilize exogenous choline for phosphatidylcholine (PC) synthesis when bacteria grow in the presence of choline. The Pcs seems to be a primary pathway for synthesis of this phospholipid in L. dumoffii. Structurally different PC species were distributed in the outer and inner membranes. As shown by the LC/ESI-MS analyses, PC15:0/15:0, PC16:0/15:0, and PC17:0/17:1 were identified in the outer membrane and PC14:0/16:0, PC16:0/17:1, and PC20:0/15:0 in the inner membrane. L. dumoffii pcsA gene encoding phosphatidylcholine synthase revealed the highest sequence identity to pcsA of L. bozemanae (82%) and L. longbeachae (81%) and lower identity to pcsA of L. drancourtii (78%) and L. pneumophila (71%). The level of TNF-α in THP1-differentiated cells induced by live and temperature-killed L. dumoffii cultured on a medium supplemented with choline was assessed. Live L. dumoffii bacteria cultured on the choline-supplemented medium induced TNF-α three-fold less efficiently than cells grown on the non-supplemented medium. There is an evident effect of PC modification, which impairs the macrophage inflammatory response. View Full-Text
Keywords: LC/ESI-MS; MALDI-TOF; Legionella dumoffii; phosphatidylcholine; TNF-α LC/ESI-MS; MALDI-TOF; Legionella dumoffii; phosphatidylcholine; TNF-α
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Palusinska-Szysz, M.; Szuster-Ciesielska, A.; Kania, M.; Janczarek, M.; Chmiel, E.; Danikiewicz, W. Legionella dumoffii Utilizes Exogenous Choline for Phosphatidylcholine Synthesis. Int. J. Mol. Sci. 2014, 15, 8256-8279.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top