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Int. J. Mol. Sci. 2014, 15(5), 8280-8292; doi:10.3390/ijms15058280

Effect of Black Soybean Koji Extract on Glucose Utilization and Adipocyte Differentiation in 3T3-L1 Cells

1
Graduate Institute of Sports Science, National Taiwan Sport University, Taoyuan 33301, Taiwan
2
Graduate Institute of Athletics and Coaching Science, National Taiwan Sport University, Taoyuan 33301, Taiwan
3
Department of Sports Sciences, University of Taipei, Taipei 11153, Taiwan
4
Department of Food Science, Nutrition, and Nutraceutical Biotechnology, Shih Chien University, Taipei 10462, Taiwan
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 13 March 2014 / Revised: 4 April 2014 / Accepted: 5 May 2014 / Published: 9 May 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Adipocyte differentiation and the extent of subsequent fat accumulation are closely related to the occurrence and progression of diseases such as insulin resistance and obesity. Black soybean koji (BSK) is produced by the fermentation of black soybean with Aspergilllus awamori. Previous study indicated that BSK extract has antioxidative and multifunctional bioactivities, however, the role of BSK in the regulation of energy metabolism is still unclear. We aimed to investigate the effect of glucose utilization on insulin-resistant 3T3-L1 preadipocytes and adipogenesis-related protein expression in differentiated adipocytes with BSK treatment. Cytoxicity assay revealed that BSK did not adversely affect cell viability at levels up to 200 µg/mL. The potential for glucose utilization was increased by increased glucose transporter 1 (GLUT1), GLUT4 and protein kinase B (AKT) protein expression in insulin-resistant 3T3-L1 cells in response to BSK treatment. Simultaneously, BSK inhibited lipid droplet accumulation in differentiated 3T3-L1 cells. The inhibitory effect of adipogenesis was associated with downregulated peroxisome proliferator-activated receptor g (PPARγ) level and upregulated Acrp30 protein expression. Our results suggest that BSK extract could improve glucose uptake by modulating GLUT1 and GLUT4 expression in a 3T3-L1 insulin-resistance cell model. In addition, BSK suppressed differentiation and lipid accumulation in mature 3T3-L1 adipocytes, which may suggest its potential for food supplementation to prevent obesity and related metabolic abnormalities. View Full-Text
Keywords: black soybean koji; 3T3-L1 preadipocytes; insulin; adipokines black soybean koji; 3T3-L1 preadipocytes; insulin; adipokines
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Huang, C.-C.; Huang, W.-C.; Hou, C.-W.; Chi, Y.-W.; Huang, H.-Y. Effect of Black Soybean Koji Extract on Glucose Utilization and Adipocyte Differentiation in 3T3-L1 Cells. Int. J. Mol. Sci. 2014, 15, 8280-8292.

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