Int. J. Mol. Sci. 2014, 15(5), 7266-7280; doi:10.3390/ijms15057266
Review

Recent Advances in Bacteria Identification by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Using Nanomaterials as Affinity Probes

email
Received: 5 March 2014; in revised form: 14 April 2014 / Accepted: 16 April 2014 / Published: 28 April 2014
(This article belongs to the Special Issue Mass Spectrometry Application in Biology)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Identifying trace amounts of bacteria rapidly, accurately, selectively, and with high sensitivity is important to ensuring the safety of food and diagnosing infectious bacterial diseases. Microbial diseases constitute the major cause of death in many developing and developed countries of the world. The early detection of pathogenic bacteria is crucial in preventing, treating, and containing the spread of infections, and there is an urgent requirement for sensitive, specific, and accurate diagnostic tests. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is an extremely selective and sensitive analytical tool that can be used to characterize different species of pathogenic bacteria. Various functionalized or unmodified nanomaterials can be used as affinity probes to capture and concentrate microorganisms. Recent developments in bacterial detection using nanomaterials-assisted MALDI-MS approaches are highlighted in this article. A comprehensive table listing MALDI-MS approaches for identifying pathogenic bacteria, categorized by the nanomaterials used, is provided.
Keywords: affinity probes; bacteria; matrix-assisted laser desorption/ionization mass spectrometry; nanomaterials
PDF Full-text Download PDF Full-Text [481 KB, uploaded 19 June 2014 05:39 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Chiu, T.-C. Recent Advances in Bacteria Identification by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Using Nanomaterials as Affinity Probes. Int. J. Mol. Sci. 2014, 15, 7266-7280.

AMA Style

Chiu T-C. Recent Advances in Bacteria Identification by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Using Nanomaterials as Affinity Probes. International Journal of Molecular Sciences. 2014; 15(5):7266-7280.

Chicago/Turabian Style

Chiu, Tai-Chia. 2014. "Recent Advances in Bacteria Identification by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Using Nanomaterials as Affinity Probes." Int. J. Mol. Sci. 15, no. 5: 7266-7280.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert