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Int. J. Mol. Sci. 2014, 15(5), 8186-8215; doi:10.3390/ijms15058186
Review

Transport Pathways—Proton Motive Force Interrelationship in Durum Wheat Mitochondria

1
, 2
, 2
 and 2,*
Received: 27 February 2014; in revised form: 18 April 2014 / Accepted: 24 April 2014 / Published: 9 May 2014
(This article belongs to the Special Issue Plant Cell Compartmentation and Volume Control)
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Abstract: In durum wheat mitochondria (DWM) the ATP-inhibited plant mitochondrial potassium channel (PmitoKATP) and the plant uncoupling protein (PUCP) are able to strongly reduce the proton motive force (pmf) to control mitochondrial production of reactive oxygen species; under these conditions, mitochondrial carriers lack the driving force for transport and should be inactive. However, unexpectedly, DWM uncoupling by PmitoKATP neither impairs the exchange of ADP for ATP nor blocks the inward transport of Pi and succinate. This uptake may occur via the plant inner membrane anion channel (PIMAC), which is physiologically inhibited by membrane potential, but unlocks its activity in de-energized mitochondria. Probably, cooperation between PIMAC and carriers may accomplish metabolite movement across the inner membrane under both energized and de-energized conditions. PIMAC may also cooperate with PmitoKATP to transport ammonium salts in DWM. Interestingly, this finding may trouble classical interpretation of in vitro mitochondrial swelling; instead of free passage of ammonia through the inner membrane and proton symport with Pi, that trigger metabolite movements via carriers, transport of ammonium via PmitoKATP and that of the counteranion via PIMAC may occur. Here, we review properties, modulation and function of the above reported DWM channels and carriers to shed new light on the control that they exert on pmf and vice-versa.
Keywords: plant mitochondria; potassium channel; anion channel; ADP/ATP carrier; uncoupling protein; mitochondrial carriers; membrane potential; reactive oxygen species plant mitochondria; potassium channel; anion channel; ADP/ATP carrier; uncoupling protein; mitochondrial carriers; membrane potential; reactive oxygen species
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Trono, D.; Laus, M.N.; Soccio, M.; Pastore, D. Transport Pathways—Proton Motive Force Interrelationship in Durum Wheat Mitochondria. Int. J. Mol. Sci. 2014, 15, 8186-8215.

AMA Style

Trono D, Laus MN, Soccio M, Pastore D. Transport Pathways—Proton Motive Force Interrelationship in Durum Wheat Mitochondria. International Journal of Molecular Sciences. 2014; 15(5):8186-8215.

Chicago/Turabian Style

Trono, Daniela; Laus, Maura N.; Soccio, Mario; Pastore, Donato. 2014. "Transport Pathways—Proton Motive Force Interrelationship in Durum Wheat Mitochondria." Int. J. Mol. Sci. 15, no. 5: 8186-8215.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert