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Int. J. Mol. Sci. 2014, 15(6), 11030-11039; doi:10.3390/ijms150611030

Contribution of Chitinase A’s C-Terminal Vacuolar Sorting Determinant to the Study of Soluble Protein Compartmentation

1
Laboratory of Cell and Molecular Biology, University of Neuchâtel, Rue Emile-Argand 11, Neuchâtel CH-2000, Switzerland
2
Institute of Biosciences and Bioresources, National Research Council of Italy (CNR–IBBR), Corso Calatafimi 414, Palermo 90129, Italy
3
ALSIA (Lucan Agency of Innovation and Technological Development)—Metapontum Agrobios Research Center, S.S. jonica 106, km 448.2, Metaponto 75012, Italy
4
DiSTeBA (Department of Biological and Environmental Sciences and Technologies), University of Salento, Campus ECOTEKNE, S.P. 6, Lecce-Monteroni, Lecce 73100, Italy
*
Author to whom correspondence should be addressed.
Received: 28 March 2014 / Revised: 6 June 2014 / Accepted: 9 June 2014 / Published: 18 June 2014
(This article belongs to the Special Issue Plant Cell Compartmentation and Volume Control)
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Abstract

Plant chitinases have been studied for their importance in the defense of crop plants from pathogen attacks and for their peculiar vacuolar sorting determinants. A peculiarity of the sequence of many family 19 chitinases is the presence of a C-terminal extension that seems to be important for their correct recognition by the vacuole sorting machinery. The 7 amino acids long C-terminal vacuolar sorting determinant (CtVSD) of tobacco chitinase A is necessary and sufficient for the transport to the vacuole. This VSD shares no homology with other CtVSDs such as the phaseolin’s tetrapeptide AFVY (AlaPheValTyr) and it is also sorted by different mechanisms. While a receptor for this signal has not yet been convincingly identified, the research using the chitinase CtVSD has been very informative, leading to the observation of phenomena otherwise difficult to observe such as the presence of separate vacuoles in differentiating cells and the existence of a Golgi-independent route to the vacuole. Thanks to these new insights in the endoplasmic reticulum (ER)-to-vacuole transport, GFPChi (Green Fluorescent Protein carrying the chitinase A CtVSD) and other markers based on chitinase signals will continue to help the investigation of vacuolar biogenesis in plants. View Full-Text
Keywords: chitinase; GFPChi; vacuole sorting; endoplasmic-to-vacuole (ERV)-pathway chitinase; GFPChi; vacuole sorting; endoplasmic-to-vacuole (ERV)-pathway
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Stigliano, E.; Di Sansebastiano, G.-P.; Neuhaus, J.-M. Contribution of Chitinase A’s C-Terminal Vacuolar Sorting Determinant to the Study of Soluble Protein Compartmentation. Int. J. Mol. Sci. 2014, 15, 11030-11039.

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