Next Article in Journal
Previous Article in Journal
Int. J. Mol. Sci. 2014, 15(5), 7313-7331; doi:10.3390/ijms15057313
Article

Genome-Wide Identification, 3D Modeling, Expression and Enzymatic Activity Analysis of Cell Wall Invertase Gene Family from Cassava (Manihot esculenta Crantz)

1,†
, 1,†
, 2
, 1
, 1
, 2,*  and 1,*
Received: 12 February 2014; in revised form: 9 April 2014 / Accepted: 14 April 2014 / Published: 28 April 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
View Full-Text   |   Download PDF [1529 KB, uploaded 19 June 2014]
Abstract: The cell wall invertases play a crucial role on the sucrose metabolism in plant source and sink organs. In this research, six cell wall invertase genes (MeCWINV1-6) were cloned from cassava. All the MeCWINVs contain a putative signal peptide with a predicted extracellular location. The overall predicted structures of the MeCWINV1-6 are similar to AtcwINV1. Their N-terminus domain forms a β-propeller module and three conserved sequence domains (NDPNG, RDP and WECP(V)D), in which the catalytic residues are situated in these domains; while the C-terminus domain consists of a β-sandwich module. The predicted structure of Pro residue from the WECPD (MeCWINV1, 2, 5, and 6), and Val residue from the WECVD (MeCWINV3 and 4) are different. The activity of MeCWINV1 and 3 were higher than other MeCWINVs in leaves and tubers, which suggested that sucrose was mainly catalyzed by the MeCWINV1 and 3 in the apoplastic space of cassava source and sink organs. The transcriptional levels of all the MeCWINVs and their enzymatic activity were lower in tubers than in leaves at all the stages during the cassava tuber development. It suggested that the major role of the MeCWINVs was on the regulation of carbon exportation from source leaves, and the ratio of sucrose to hexose in the apoplasts; the role of these enzymes on the sucrose unloading to tuber was weaker.
Keywords: molecular cloning; cell wall invertase; cassava; gene expression analysis; 3D modeling; enzyme activities molecular cloning; cell wall invertase; cassava; gene expression analysis; 3D modeling; enzyme activities
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Yao, Y.; Geng, M.-T.; Wu, X.-H.; Liu, J.; Li, R.-M.; Hu, X.-W.; Guo, J.-C. Genome-Wide Identification, 3D Modeling, Expression and Enzymatic Activity Analysis of Cell Wall Invertase Gene Family from Cassava (Manihot esculenta Crantz). Int. J. Mol. Sci. 2014, 15, 7313-7331.

AMA Style

Yao Y, Geng M-T, Wu X-H, Liu J, Li R-M, Hu X-W, Guo J-C. Genome-Wide Identification, 3D Modeling, Expression and Enzymatic Activity Analysis of Cell Wall Invertase Gene Family from Cassava (Manihot esculenta Crantz). International Journal of Molecular Sciences. 2014; 15(5):7313-7331.

Chicago/Turabian Style

Yao, Yuan; Geng, Meng-Ting; Wu, Xiao-Hui; Liu, Jiao; Li, Rui-Mei; Hu, Xin-Wen; Guo, Jian-Chun. 2014. "Genome-Wide Identification, 3D Modeling, Expression and Enzymatic Activity Analysis of Cell Wall Invertase Gene Family from Cassava (Manihot esculenta Crantz)." Int. J. Mol. Sci. 15, no. 5: 7313-7331.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert