Next Article in Journal
Next Article in Special Issue
Previous Article in Journal
Previous Article in Special Issue
Int. J. Mol. Sci. 2014, 15(5), 7293-7312; doi:10.3390/ijms15057293
Article

Efficient Delivery of Plasmid DNA Using Cholesterol-Based Cationic Lipids Containing Polyamines and Ether Linkages

1,2,†
, 1,†
, 2
, 1
, 1
, 3
, 1
, 2
 and 2,*
Received: 27 January 2014; in revised form: 24 March 2014 / Accepted: 10 April 2014 / Published: 28 April 2014
(This article belongs to the Special Issue Bioactive Nanoparticles 2014)
View Full-Text   |   Download PDF [1458 KB, uploaded 19 June 2014]   |   Browse Figures
Abstract: Cationic liposomes are broadly used as non-viral vectors to deliver genetic materials that can be used to treat various diseases including cancer. To circumvent problems associated with cationic liposome-mediated delivery systems such as low transfection efficiency and serum-induced inhibition, cholesterol-based cationic lipids have been synthesized that resist the effects of serum. The introduction of an ether-type linkage and extension of the aminopropyl head group on the cholesterol backbone increased the transfection efficiency and DNA binding affinity compared to a carbamoyl-type linkage and a mono aminopropyl head group, respectively. Under optimal conditions, each liposome formulation showed higher transfection efficiency in AGS and Huh-7 cells than commercially available cationic liposomes, particularly in the presence of serum. The following molecular structures were found to have a positive effect on transfection properties: (i) extended aminopropyl head groups for a strong binding affinity to plasmid DNA; (ii) an ether linkage that favors electrostatic binding to plasmid DNA; and (iii) a cholesterol backbone for serum resistance.
Keywords: cholesterol; cationic lipids; cyanoethylation; transfection; gene delivery; macropinocytosis cholesterol; cationic lipids; cyanoethylation; transfection; gene delivery; macropinocytosis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Kim, B.-K.; Seu, Y.-B.; Bae, Y.-U.; Kwak, T.-W.; Kang, H.; Moon, I.-J.; Hwang, G.-B.; Park, S.-Y.; Doh, K.-O. Efficient Delivery of Plasmid DNA Using Cholesterol-Based Cationic Lipids Containing Polyamines and Ether Linkages. Int. J. Mol. Sci. 2014, 15, 7293-7312.

AMA Style

Kim B-K, Seu Y-B, Bae Y-U, Kwak T-W, Kang H, Moon I-J, Hwang G-B, Park S-Y, Doh K-O. Efficient Delivery of Plasmid DNA Using Cholesterol-Based Cationic Lipids Containing Polyamines and Ether Linkages. International Journal of Molecular Sciences. 2014; 15(5):7293-7312.

Chicago/Turabian Style

Kim, Bieong-Kil; Seu, Young-Bae; Bae, Yun-Ui; Kwak, Tae-Won; Kang, Hyungu; Moon, Ik-Jae; Hwang, Guen-Bae; Park, So-Young; Doh, Kyung-Oh. 2014. "Efficient Delivery of Plasmid DNA Using Cholesterol-Based Cationic Lipids Containing Polyamines and Ether Linkages." Int. J. Mol. Sci. 15, no. 5: 7293-7312.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert