Int. J. Mol. Sci. 2014, 15(3), 5063-5078; doi:10.3390/ijms15035063
Article

A Chrysanthemum Heat Shock Protein Confers Tolerance to Abiotic Stress

1,2,†email, 1,2,†email, 1email, 1email, 1email and 1,2,* email
Received: 15 December 2013; in revised form: 12 March 2014 / Accepted: 13 March 2014 / Published: 21 March 2014
(This article belongs to the Special Issue Plant Cell Compartmentation and Volume Control)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Heat shock proteins are associated with protection against various abiotic stresses. Here, the isolation of a chrysanthemum cDNA belonging to the HSP70 family is reported. The cDNA, designated CgHSP70, encodes a 647-residue polypeptide, of estimated molecular mass 70.90 kDa and pI 5.12. A sub-cellular localization assay indicated that the cDNA product is deposited in the cytoplasm and nucleus. The performance of Arabidopsis thaliana plants constitutively expressing CgHSP70 demonstrated that the gene enhances tolerance to heat, drought and salinity. When CgHSP70 was stably over-expressed in chrysanthemum, the plants showed an increased peroxidase (POD) activity, higher proline content and inhibited malondialdehyde (MDA) content. After heat stress, drought or salinity the transgenic plants were better able to recover, demonstrating CgHSP70 positive effect.
Keywords: abiotic stress tolerance; heat shock protein; transformation
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MDPI and ACS Style

Song, A.; Zhu, X.; Chen, F.; Gao, H.; Jiang, J.; Chen, S. A Chrysanthemum Heat Shock Protein Confers Tolerance to Abiotic Stress. Int. J. Mol. Sci. 2014, 15, 5063-5078.

AMA Style

Song A, Zhu X, Chen F, Gao H, Jiang J, Chen S. A Chrysanthemum Heat Shock Protein Confers Tolerance to Abiotic Stress. International Journal of Molecular Sciences. 2014; 15(3):5063-5078.

Chicago/Turabian Style

Song, Aiping; Zhu, Xirong; Chen, Fadi; Gao, Haishun; Jiang, Jiafu; Chen, Sumei. 2014. "A Chrysanthemum Heat Shock Protein Confers Tolerance to Abiotic Stress." Int. J. Mol. Sci. 15, no. 3: 5063-5078.

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