Abstract: We investigated the effect of auxin and acetylcholine on the expression of the tomato expansin gene LeEXPA2, a specific expansin gene expressed in elongating tomato hypocotyl segments. Since auxin interferes with clathrin-mediated endocytosis, in order to regulate cellular and developmental responses we produced protoplasts from tomato elongating hypocotyls and followed the endocytotic marker, FM4-64, internalization in response to treatments. Tomato protoplasts were observed during auxin and acetylcholine treatments after transient expression of chimerical markers of volume-control related compartments such as vacuoles. Here we describe the contribution of auxin and acetylcholine to LeEXPA2 expression regulation and we support the hypothesis that a possible subcellular target of acetylcholine signal is the vesicular transport, shedding some light on the characterization of this small molecule as local mediator in the plant physiological response.
Keywords: cell elongation; acetylcholine; expansin; protoplasts; endocytosis; vesicle trafficking; vacuoles
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Di Sansebastiano, G.-P.; Fornaciari, S.; Barozzi, F.; Piro, G.; Arru, L. New Insights on Plant Cell Elongation: A Role for Acetylcholine. Int. J. Mol. Sci. 2014, 15, 4565-4582.
Di Sansebastiano G-P, Fornaciari S, Barozzi F, Piro G, Arru L. New Insights on Plant Cell Elongation: A Role for Acetylcholine. International Journal of Molecular Sciences. 2014; 15(3):4565-4582.
Di Sansebastiano, Gian-Pietro; Fornaciari, Silvia; Barozzi, Fabrizio; Piro, Gabriella; Arru, Laura. 2014. "New Insights on Plant Cell Elongation: A Role for Acetylcholine." Int. J. Mol. Sci. 15, no. 3: 4565-4582.