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Int. J. Mol. Sci. 2014, 15(3), 4565-4582; doi:10.3390/ijms15034565

New Insights on Plant Cell Elongation: A Role for Acetylcholine

1
DiSTeBA (Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali), University of Salento, Campus ECOTEKNE, Lecce 73100, Italy
2
Interdepartmental Research Centre Biogest-Siteia, University of Modena and Reggio Emilia, Reggio Emilia 42122, Italy
3
Department of Life Science, University of Modena and Reggio Emilia, Reggio Emilia 42122, Italy
*
Author to whom correspondence should be addressed.
Received: 12 February 2014 / Revised: 7 March 2014 / Accepted: 11 March 2014 / Published: 17 March 2014
(This article belongs to the Special Issue Plant Cell Compartmentation and Volume Control)
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Abstract

We investigated the effect of auxin and acetylcholine on the expression of the tomato expansin gene LeEXPA2, a specific expansin gene expressed in elongating tomato hypocotyl segments. Since auxin interferes with clathrin-mediated endocytosis, in order to regulate cellular and developmental responses we produced protoplasts from tomato elongating hypocotyls and followed the endocytotic marker, FM4-64, internalization in response to treatments. Tomato protoplasts were observed during auxin and acetylcholine treatments after transient expression of chimerical markers of volume-control related compartments such as vacuoles. Here we describe the contribution of auxin and acetylcholine to LeEXPA2 expression regulation and we support the hypothesis that a possible subcellular target of acetylcholine signal is the vesicular transport, shedding some light on the characterization of this small molecule as local mediator in the plant physiological response.
Keywords: cell elongation; acetylcholine; expansin; protoplasts; endocytosis; vesicle trafficking; vacuoles cell elongation; acetylcholine; expansin; protoplasts; endocytosis; vesicle trafficking; vacuoles
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MDPI and ACS Style

Di Sansebastiano, G.-P.; Fornaciari, S.; Barozzi, F.; Piro, G.; Arru, L. New Insights on Plant Cell Elongation: A Role for Acetylcholine. Int. J. Mol. Sci. 2014, 15, 4565-4582.

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