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Int. J. Mol. Sci. 2014, 15(3), 4565-4582; doi:10.3390/ijms15034565
Article

New Insights on Plant Cell Elongation: A Role for Acetylcholine

1
, 2
, 3
, 1
 and 2,3,*
1 DiSTeBA (Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali), University of Salento, Campus ECOTEKNE, Lecce 73100, Italy 2 Interdepartmental Research Centre Biogest-Siteia, University of Modena and Reggio Emilia, Reggio Emilia 42122, Italy 3 Department of Life Science, University of Modena and Reggio Emilia, Reggio Emilia 42122, Italy
* Author to whom correspondence should be addressed.
Received: 12 February 2014 / Revised: 7 March 2014 / Accepted: 11 March 2014 / Published: 17 March 2014
(This article belongs to the Special Issue Plant Cell Compartmentation and Volume Control)
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Abstract

We investigated the effect of auxin and acetylcholine on the expression of the tomato expansin gene LeEXPA2, a specific expansin gene expressed in elongating tomato hypocotyl segments. Since auxin interferes with clathrin-mediated endocytosis, in order to regulate cellular and developmental responses we produced protoplasts from tomato elongating hypocotyls and followed the endocytotic marker, FM4-64, internalization in response to treatments. Tomato protoplasts were observed during auxin and acetylcholine treatments after transient expression of chimerical markers of volume-control related compartments such as vacuoles. Here we describe the contribution of auxin and acetylcholine to LeEXPA2 expression regulation and we support the hypothesis that a possible subcellular target of acetylcholine signal is the vesicular transport, shedding some light on the characterization of this small molecule as local mediator in the plant physiological response.
Keywords: cell elongation; acetylcholine; expansin; protoplasts; endocytosis; vesicle trafficking; vacuoles cell elongation; acetylcholine; expansin; protoplasts; endocytosis; vesicle trafficking; vacuoles
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Di Sansebastiano, G.-P.; Fornaciari, S.; Barozzi, F.; Piro, G.; Arru, L. New Insights on Plant Cell Elongation: A Role for Acetylcholine. Int. J. Mol. Sci. 2014, 15, 4565-4582.

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