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Int. J. Mol. Sci. 2014, 15(3), 4733-4746; doi:10.3390/ijms15034733
Article

Nitric Oxide Functions as a Signal in Ultraviolet-B-Induced Baicalin Accumulation in Scutellaria baicalensis Suspension Cultures

1
, 2
, 3,*  and 2,*
Received: 24 January 2014; in revised form: 26 February 2014 / Accepted: 11 March 2014 / Published: 18 March 2014
(This article belongs to the Special Issue Signalling Molecules and Signal Transduction in Cells 2014)
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Abstract: Stress induced by ultraviolet-B (UV-B) irradiation stimulates the accumulation of various secondary metabolites in plants. Nitric oxide (NO) serves as an important secondary messenger in UV-B stress-induced signal transduction pathways. NO can be synthesized in plants by either enzymatic catalysis or an inorganic nitrogen pathway. The effects of UV-B irradiation on the production of baicalin and the associated molecular pathways in plant cells are poorly understood. In this study, nitric oxide synthase (NOS) activity, NO release and the generation of baicalin were investigated in cell suspension cultures of Scutellaria baicalensis exposed to UV-B irradiation. UV-B irradiation significantly increased NOS activity, NO release and baicalin biosynthesis in S. baicalensis cells. Additionally, exogenous NO supplied by the NO donor, sodium nitroprusside (SNP), led to a similar increase in the baicalin content as the UV-B treatment. The NOS inhibitor, Nω-nitro-l-arginine (LNNA), and NO scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) partially inhibited UV-B-induced NO release and baicalin accumulation. These results suggest that NO is generated by NOS or NOS-like enzymes and plays an important role in baicalin biosynthesis as part of the defense response of S. baicalensis cells to UV-B irradiation.
Keywords: baicalin; nitric oxide; NOS activity; Scutellaria baicalensis; UV-B irradiation baicalin; nitric oxide; NOS activity; Scutellaria baicalensis; UV-B irradiation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Zhang, J.-J.; Li, X.-Q.; Sun, J.-W.; Jin, S.-H. Nitric Oxide Functions as a Signal in Ultraviolet-B-Induced Baicalin Accumulation in Scutellaria baicalensis Suspension Cultures. Int. J. Mol. Sci. 2014, 15, 4733-4746.

AMA Style

Zhang J-J, Li X-Q, Sun J-W, Jin S-H. Nitric Oxide Functions as a Signal in Ultraviolet-B-Induced Baicalin Accumulation in Scutellaria baicalensis Suspension Cultures. International Journal of Molecular Sciences. 2014; 15(3):4733-4746.

Chicago/Turabian Style

Zhang, Jin-Jie; Li, Xue-Qin; Sun, Jun-Wei; Jin, Song-Heng. 2014. "Nitric Oxide Functions as a Signal in Ultraviolet-B-Induced Baicalin Accumulation in Scutellaria baicalensis Suspension Cultures." Int. J. Mol. Sci. 15, no. 3: 4733-4746.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert