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Int. J. Mol. Sci. 2014, 15(3), 3871-3888; doi:10.3390/ijms15033871
Article

Isolation and Characterization of the Brassinosteroid Receptor Gene (GmBRI1) from Glycine max

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, 2
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, 2,*  and 1,*
Received: 1 December 2013; in revised form: 17 February 2014 / Accepted: 17 February 2014 / Published: 4 March 2014
(This article belongs to the Special Issue Plant Cell Compartmentation and Volume Control)
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Abstract: Brassinosteroids (BRs) constitute a group of steroidal phytohormones that contribute to a wide range of plant growth and development functions. The genetic modulation of BR receptor genes, which play major roles in the BR signaling pathway, can create semi-dwarf plants that have great advantages in crop production. In this study, a brassinosteroid insensitive gene homologous with AtBRI1 and other BRIs was isolated from Glycine max and designated as GmBRI1. A bioinformatic analysis revealed that GmBRI1 shares a conserved kinase domain and 25 tandem leucine-rich repeats (LRRs) that are characteristic of a BR receptor for BR reception and reaction and bear a striking similarity in protein tertiary structure to AtBRI1. GmBRI1 transcripts were more abundant in soybean hypocotyls and could be upregulated in response to exogenous BR treatment. The transformation of GmBRI1 into the Arabidopsis dwarf mutant bri1-5 restored the phenotype, especially regarding pod size and plant height. Additionally, this complementation is a consequence of a restored BR signaling pathway demonstrated in the light/dark analysis, root inhibition assay and BR-response gene expression. Therefore, GmBRI1 functions as a BR receptor to alter BR-mediated signaling and is valuable for improving plant architecture and enhancing the yield of soybean.
Keywords: brassinosteroids; GmBRI1; BR receptor; yield; soybean; BR signal transduction brassinosteroids; GmBRI1; BR receptor; yield; soybean; BR signal transduction
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Wang, M.; Sun, S.; Wu, C.; Han, T.; Wang, Q. Isolation and Characterization of the Brassinosteroid Receptor Gene (GmBRI1) from Glycine max. Int. J. Mol. Sci. 2014, 15, 3871-3888.

AMA Style

Wang M, Sun S, Wu C, Han T, Wang Q. Isolation and Characterization of the Brassinosteroid Receptor Gene (GmBRI1) from Glycine max. International Journal of Molecular Sciences. 2014; 15(3):3871-3888.

Chicago/Turabian Style

Wang, Miao; Sun, Shi; Wu, Cunxiang; Han, Tianfu; Wang, Qingyu. 2014. "Isolation and Characterization of the Brassinosteroid Receptor Gene (GmBRI1) from Glycine max." Int. J. Mol. Sci. 15, no. 3: 3871-3888.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert