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Int. J. Mol. Sci. 2014, 15(3), 4299-4317; doi:10.3390/ijms15034299

The Effect of Physical and Chemical Cues on Hepatocellular Function and Morphology

Received: 18 November 2013 / Revised: 14 February 2014 / Accepted: 25 February 2014 / Published: 11 March 2014
(This article belongs to the Special Issue Interaction between Nano-Structure Materials and Cells)
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Physical topographical features and/or chemical stimuli to the extracellular matrix (ECM) provide essential cues that manipulate cell functions. From the physical point of view, contoured nanostructures are very important for cell behavior in general, and for cellular functions. From the chemical point of view, ECM proteins containing an RGD sequence are known to alter cell functions. In this study, the influence of integrated physical and chemical cues on a liver cell line (HepG2) was investigated. To mimic the physical cues provided by the ECM, amorphous TiO2 nanogratings with specific dimensional and geometrical characteristics (nanogratings 90 nm wide and 150 nm apart) were fabricated. To mimic the chemical cues provided by the ECM, the TiO2 inorganic film was modified by immobilization of the RGD motif. The hepatic cell line morphological and functional changes induced by simultaneously combining these diversified cues were investigated, including cellular alignment and the expression of different functional proteins. The combination of nanopatterns and surface modification with RGD induced cellular alignment and expression of functional proteins, indicating that physical and chemical cues are important factors for optimizing hepatocyte function.
Keywords: hepatocytes; topography; RGD; TiO2 hepatocytes; topography; RGD; TiO2
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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Abdellatef, S.A.; Ohi, A.; Nabatame, T.; Taniguchi, A. The Effect of Physical and Chemical Cues on Hepatocellular Function and Morphology. Int. J. Mol. Sci. 2014, 15, 4299-4317.

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