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Int. J. Mol. Sci. 2014, 15(3), 4060-4076; doi:10.3390/ijms15034060
Article

Long Non-Coding RNA HOTAIR Promotes Cell Migration and Invasion via Down-Regulation of RNA Binding Motif Protein 38 in Hepatocellular Carcinoma Cells

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Received: 2 January 2014; in revised form: 20 February 2014 / Accepted: 27 February 2014 / Published: 6 March 2014
(This article belongs to the collection Molecular Mechanisms of Human Liver Diseases)
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Abstract: Long non-coding RNA HOTAIR exerts regulatory functions in various biological processes in cancer cells, such as proliferation, apoptosis, mobility, and invasion. We previously found that HOX transcript antisense RNA (HOTAIR) is a negative prognostic factor and exhibits oncogenic activity in hepatocellular carcinoma (HCC). In this study, we aimed to investigate the role and molecular mechanism of HOTAIR in promoting HCC cell migration and invasion. Firstly, we profiled its gene expression pattern by microarray analysis of HOTAIR loss in Bel-7402 HCC cell line. The results showed that 129 genes were significantly down-regulated, while 167 genes were significantly up-regulated (fold change >2, p < 0.05). Bioinformatics analysis indicated that RNA binding proteins were involved in this biological process. HOTAIR suppression using RNAi strategy with HepG2 and Bel-7402 cells increased the mRNA and protein expression levels of RNA binding motif protein 38 (RBM38). Moreover, the expression levels of RBM38 in HCC specimens were significantly lower than paired adjacent noncancerous tissues. In addition, knockdown of HOTAIR resulted in a decrease of cell migration and invasion, which could be specifically rescued by down-regulation of RBM38. Taken together, HOTAIR could promote migration and invasion of HCC cells by inhibiting RBM38, which indicated critical roles of HOTAIR and RBM38 in HCC progression.
Keywords: RNA binding motif protein 38; HOX transcript antisense RNA; hepatocellular carcinoma; long non-coding RNA RNA binding motif protein 38; HOX transcript antisense RNA; hepatocellular carcinoma; long non-coding RNA
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Ding, C.; Cheng, S.; Yang, Z.; Lv, Z.; Xiao, H.; Du, C.; Peng, C.; Xie, H.; Zhou, L.; Wu, J.; Zheng, S. Long Non-Coding RNA HOTAIR Promotes Cell Migration and Invasion via Down-Regulation of RNA Binding Motif Protein 38 in Hepatocellular Carcinoma Cells. Int. J. Mol. Sci. 2014, 15, 4060-4076.

AMA Style

Ding C, Cheng S, Yang Z, Lv Z, Xiao H, Du C, Peng C, Xie H, Zhou L, Wu J, Zheng S. Long Non-Coding RNA HOTAIR Promotes Cell Migration and Invasion via Down-Regulation of RNA Binding Motif Protein 38 in Hepatocellular Carcinoma Cells. International Journal of Molecular Sciences. 2014; 15(3):4060-4076.

Chicago/Turabian Style

Ding, Chaofeng; Cheng, Shaobing; Yang, Zhe; Lv, Zhen; Xiao, Heng; Du, Chengli; Peng, Chuanhui; Xie, Haiyang; Zhou, Lin; Wu, Jian; Zheng, Shusen. 2014. "Long Non-Coding RNA HOTAIR Promotes Cell Migration and Invasion via Down-Regulation of RNA Binding Motif Protein 38 in Hepatocellular Carcinoma Cells." Int. J. Mol. Sci. 15, no. 3: 4060-4076.



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