Next Article in Journal
The Effect and Mechanism of Tamoxifen-Induced Hepatocyte Steatosis in Vitro
Previous Article in Journal
Increased Circulatory Asymmetric Dimethylarginine and Multiple Organ Failure: Bile Duct Ligation in Rat as a Model
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2014, 15(3), 4007-4018; doi:10.3390/ijms15034007

miR-21 Promotes Human Nucleus Pulposus Cell Proliferation through PTEN/AKT Signaling

Department of Orthopedic Surgery, Hunan Provincial People's Hospital, Changsha 10005, China
*
Author to whom correspondence should be addressed.
Received: 24 November 2013 / Revised: 16 January 2014 / Accepted: 26 January 2014 / Published: 5 March 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
View Full-Text   |   Download PDF [1620 KB, uploaded 19 June 2014]   |  

Abstract

The precise role of nucleus pulposus cell proliferation in the pathogenesis of intervertebral disc degeneration remains to be elucidated. Recent findings have revealed that microRNAs, a class of small noncoding RNAs, may regulate cell proliferation in many pathological conditions. Here, we showed that miR-21 was significantly upregulated in degenerative nucleus pulposus tissues when compared with nucleus pulposus tissues that were isolated from patients with idiopathic scoliosis and that miR-10b levels were associated with disc degeneration grade. Moreover, bioinformatics target prediction identified PTEN as a putative target of miR-21. miR-21 inhibited PTEN expression by directly targeting the 3'UTR, and this inhibition was abolished through miR-21 binding site mutations. miR-21 overexpression stimulated cell proliferation and AKT signaling pathway activation, which led to cyclin D1 translation. Additionally, the increase in proliferation and cyclin D1 expression induced by miR-21 overexpression was almost completely blocked by Ly294002, an AKT inhibitor. Taken together, aberrant miR-21 upregulation in intervertebral disc degeneration could target PTEN, which would contribute to abnormal nucleus pulposus cell proliferation through derepressing the Akt pathway. Our study also underscores the potential of miR-21 and the PTEN/Akt pathway as novel therapeutic targets in intervertebral disc degeneration.
Keywords: disc degeneration; nucleus pulposus cells; miRNA; miR-21; proliferation disc degeneration; nucleus pulposus cells; miRNA; miR-21; proliferation
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Liu, H.; Huang, X.; Liu, X.; Xiao, S.; Zhang, Y.; Xiang, T.; Shen, X.; Wang, G.; Sheng, B. miR-21 Promotes Human Nucleus Pulposus Cell Proliferation through PTEN/AKT Signaling. Int. J. Mol. Sci. 2014, 15, 4007-4018.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top