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Int. J. Mol. Sci. 2014, 15(3), 3729-3745; doi:10.3390/ijms15033729

Effect of NK4 Transduction in Bone Marrow-Derived Mesenchymal Stem Cells on Biological Characteristics of Pancreatic Cancer Cells

1
Department of Hepatobiliary Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, China
2
Department of General Surgery, Yiwu Chouzhou Hospital, Yiwu 322000, Zhejiang, China
3
Department of Infectious Disease, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, China
*
Author to whom correspondence should be addressed.
Received: 28 November 2013 / Revised: 20 January 2014 / Accepted: 10 February 2014 / Published: 3 March 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Pancreatic cancer usually has a poor prognosis, and no gene therapy has yet been developed that is effective to treat it. Since a unique characteristic of bone marrow-derived mesenchymal stem cells (MSCs) is that they migrate to tumor tissues, we wanted to determine whether MSCs could serve as a vehicle of gene therapy for targeting pancreatic cancer. First, we successfully extracted MSCs from SD rats. Next, MSCs were efficiently transduced with NK4, an antagonist of hepatocyte growth factor (HGF) which comprising the N-terminal and the subsequent four kringle domains of HGF, by an adenoviral vector. Then, we confirmed that rat MSCs preferentially migrate to pancreatic cancer cells. Last, MSCs expressing NK4 (NK4-MSCs) strongly inhibited proliferation and migration of the pancreatic cancer cell line SW1990 after co-culture. These results indicate that MSCs can serve as a vehicle of gene therapy for targeting pancreatic cancer. View Full-Text
Keywords: NK4; pancreatic cancer; bone marrow-derived mesenchymal stem cells; transduction NK4; pancreatic cancer; bone marrow-derived mesenchymal stem cells; transduction
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Sun, Y.-P.; Zhang, B.-L.; Duan, J.-W.; Wu, H.-H.; Wang, B.-Q.; Yu, Z.-P.; Yang, W.-J.; Shan, Y.-F.; Zhou, M.-T.; Zhang, Q.-Y. Effect of NK4 Transduction in Bone Marrow-Derived Mesenchymal Stem Cells on Biological Characteristics of Pancreatic Cancer Cells. Int. J. Mol. Sci. 2014, 15, 3729-3745.

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