Association between Single Nucleotide Polymorphism rs1044925 and the Risk of Coronary Artery Disease and Ischemic Stroke
Abstract
:1. Introduction
2. Results
2.1. General Characteristics and Serum Lipid Levels
2.2. Genotypic and Allelic Frequencies
2.3. ACAT-1 SNP rs1044925 and the Risk of CAD and IS
2.4. Related Risk Factors for CAD and IS
2.5. Genotypes and Serum Lipid Levels
3. Discussion
4. Materials and Methods
4.1. Cases and Controls
4.2. Genotyping and Biochemical Analysis
4.3. Diagnostic Criteria
4.4. Statistical Analyses
5. Conclusions
Acknowledgments
Conflicts of Interest
References
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Parameter | Control | CAD | IS | P1 | P2 |
---|---|---|---|---|---|
Number | 588 | 587 | 555 | – | – |
Male/female | 427/161 | 433/154 | 400/155 | 0.674 | 0.805 |
Age (years) | 61.51 ± 10.85 | 62.25 ± 10.55 | 62.85 ± 12.33 | 0.236 | 0.052 |
Body mass index (kg/m2) | 22.42 ± 2.85 | 24.03 ± 3.20 | 23.41 ± 3.52 | <0.001 | <0.001 |
Systolic blood pressure (mmHg) | 130.45 ± 20.13 | 133.32 ± 23.56 | 147.74 ± 22.08 | 0.026 | <0.001 |
Diastolic blood pressure (mmHg) | 82.23 ± 13.24 | 79.37 ± 14.28 | 83.69 ± 12.77 | <0.001 | 0.059 |
Pulse pressure (mmHg) | 49.84 ± 15.06 | 53.49 ± 18.31 | 63.89 ± 18.25 | <0.001 | <0.001 |
Cigarette smoking (n (%)) | 258 (43.9) | 274 (46.7) | 224 (40.4) | 0.335 | 0.253 |
Alcohol consumption (n (%)) | 267 (45.4) | 159 (27.1) | 166 (29.9) | <0.001 | <0.001 |
Total cholesterol (mmol/L) | 4.93 ± 1.04 | 4.54 ± 1.22 | 4.53 ± 1.14 | <0.001 | <0.001 |
Triglyceride (mmol/L) | 1.41 ± 1.75 | 1.67 ± 1.13 | 1.68 ± 1.37 | 0.004 | 0.005 |
HDL-C (mmol/L) | 1.88 ± 0.50 | 1.15 ± 0.34 | 1.23 ± 0.41 | <0.001 | <0.001 |
LDL-C (mmol/L) | 2.74 ± 0.79 | 2.71 ± 1.03 | 2.68 ± 0.90 | 0.489 | 0.238 |
Apolipoprotein (Apo) AI (g/L) | 1.41 ± 0.27 | 1.05 ± 0.55 | 1.03 ± 0.25 | <0.001 | <0.001 |
ApoB (g/L) | 0.89 ± 0.21 | 0.91 ± 0.27 | 0.89 ± 0.25 | 0.376 | 0.992 |
ApoAI/ApoB | 1.67 ± 0.59 | 1.25 ± 0.82 | 1.19 ± 0.60 | <0.001 | <0.001 |
Genotype or allele | Control (n (%)) | CAD (n (%)) | IS (n (%)) | CAD | IS | ||
---|---|---|---|---|---|---|---|
n = 588 | n = 587 | n = 555 | OR (95% CI) | p | OR (95% CI) | p | |
AA | 412 (70.1) | 452 (77.0) | 424 (76.4) | 1 | 1 | ||
AC | 166 (28.2) | 123 (21.0) | 125 (22.5) | 0.67 (0.50–0.91) | 0.009 | 0.72 (0.54–0.95) | 0.021 |
CC | 10 (1.7) | 12 (2.0) | 6 (1.1) | 0.95 (0.36–2.53) | 0.921 | 0.56 (0.20–1.57) | 0.269 |
χ2 | 8.431 | 6.001 | |||||
P | 0.015 | 0.050 | |||||
AA | 412 (70.1) | 452 (77.0) | 424 (76.4) | 1 | 1 | ||
AC + CC | 176 (29.9) | 135 (23.0) | 131 (23.6) | 0.69 (0.52–0.93) | 0.014 | 0.71 (0.54–0.93) | 0.014 |
χ2 | 7.256 | 5.820 | |||||
P | 0.007 | 0.016 | |||||
A | 990 (84.2) | 1027 (87.5) | 973 (87.7) | 1 | 1 | ||
C | 186 (15.8) | 147 (12.5) | 137 (12.3) | 0.76 (0.60–0.96) | 0.022 | 0.75 (0.59–0.95) | 0.017 |
χ2 | 5.245 | 5.680 | |||||
P | 0.022 | 0.017 |
Factors | Genotype | CAD | IS | ||||
---|---|---|---|---|---|---|---|
OR (95% CI) | p | pinteraction | OR (95% CI) | p | pinteraction | ||
BMI | |||||||
<24 kg/m2 | AA | 1 | 1 | ||||
AC + CC | 0.79 (0.54–1.14) | 0.208 | 0.82 (0.58–1.14) | 0.238 | |||
≥24 kg/m2 | AA | 1 | 0.507 | 0.221 | |||
AC + CC | 0.57 (0.36–0.91) | 0.018 | 0.53 (0.34–0.84) | 0.007 | |||
Gender | |||||||
Male | AA | 1 | 1 | ||||
AC + CC | 0.71 (0.51–1.00) | 0.050 | 0.69 (0.50–0.95) | 0.024 | |||
Female | AA | 1 | 0.777 | 1 | 0.946 | ||
AC + CC | 0.67 (0.37–1.20) | 0.178 | 0.75 (0.44–1.29) | 0.295 | |||
Smoking | |||||||
Nonsmoker | AA | 1 | 1 | ||||
AC + CC | 0.63 (0.42–0.94) | 0.023 | 0.73 (0.52–1.04) | 0.078 | |||
Smoker | AA | 1 | 0.459 | 1 | 0.980 | ||
AC + CC | 0.79 (0.51–1.22) | 0.288 | 0.71 (0.42–1.09) | 0.112 | |||
Drinking | |||||||
Nondrinker | AA | 1 | 1 | ||||
AC + CC | 0.66 (0.47–0.93) | 0.017 | 0.68 (0.49–0.95) | 0.024 | |||
Drinker | AA | 1 | 0.831 | 1 | 0.709 | ||
AC + CC | 0.75 (0.44–1.28) | 0.291 | 0.75 (0.47–1.21) | 0.242 |
Relative Factors | CAD | IS | ||
---|---|---|---|---|
OR (95% CI) | p | OR (95% CI) | p | |
Nonsmoking | 1 | 1 | ||
Smoking | 1.39 (1.06–1.82) | 0.016 | 1.05 (0.80–1.38) | 0.724 |
Nondrinking | 1 | 1 | ||
Drinking | 0.29 (0.22–0.39) | <0.001 | 0.41 (0.31–0.55) | <0.001 |
BMI < 24 kg/m2 | 1 | 1 | ||
BMI ≥ 24 kg/m2 | 2.37 (1.82–3.08) | <0.001 | 1.60 (1.22–2.88) | 0.001 |
rs1044925 AA | 1 | 1 | ||
rs1044925 AC/CC | 0.71 (0.53–0.95) | 0.020 | 0.66 (0.50–0.89) | 0.005 |
Non-diabetes | 1 | 1 | ||
Diabetes | 4.50 (3.05–6.65) | <0.001 | 2.68 (1.72–3.92) | <0.001 |
Normotensive | 1 | 1 | ||
Hypertension | 2.18 (1.72–2.76) | <0.001 | 3.33 (2.57–4.32) | <0.001 |
Normal blood lipids | 1 | 1 | ||
Hyperlipidemia | 1.67 (1.32–2.11) | <0.001 | 2.03 (1.57–2.55) | <0.001 |
Genotype | n | TC (mmol/L) | TG (mmol/L) | HDL-C (mmol/L) | LDL-C (mmol/L) | ApoAI (g/L) | ApoB (g/L) | ApoAI/ApoB |
---|---|---|---|---|---|---|---|---|
Control | ||||||||
AA | 412 | 4.89 ± 0.92 | 1.29 ± 1.22 | 1.85 ± 0.48 | 2.76 ± 0.78 | 1.40 ± 0.28 | 0.89 ± 0.21 | 1.66 ± 0.63 |
AC/CC | 176 | 4.97 ± 1.24 | 1.35 ± 2.64 | 1.94 ± 0.55 | 2.71 ± 0.82 | 1.42 ± 0.25 | 0.89 ± 0.22 | 1.68 ± 0.47 |
F | – | 0.959 | 0.590 | 4.311 | 0.616 | 0.793 | 0.000 | 0.113 |
p | – | 0.328 | 0.443 | 0.038 | 0.433 | 0.373 | 0.996 | 0.737 |
CAD | ||||||||
AA | 452 | 4.51 ± 1.18 | 1.68 ± 1.18 | 1.14 ± 0.35 | 2.68 ± 1.00 | 1.03 ± 0.37 | 0.90 ± 0.26 | 1.21 ± 0.58 |
AC/CC | 135 | 4.58 ± 1.34 | 1.58 ± 1.00 | 1.18 ± 0.33 | 2.73 ± 1.08 | 1.11 ± 0.93 | 0.88 ± 0.30 | 1.37 ± 1.34 |
F | – | 0.418 | 1.109 | 0.966 | 0.256 | 2.190 | 1.728 | 3.727 |
p | – | 0.518 | 0.293 | 0.326 | 0.613 | 0.130 | 0.189 | 0.054 |
IS | ||||||||
AA | 424 | 4.53 ± 1.20 | 1.68 ± 1.47 | 1.24 ± 0.43 | 2.69 ± 0.94 | 1.03 ± 0.23 | 0.89 ± 0.25 | 1.19 ± 0.64 |
AC/CC | 131 | 4.58 ± 0.95 | 1.72 ± 1.08 | 1.17 ± 0.31 | 2.73 ± 0.76 | 1.00 ± 0.18 | 0.92 ± 0.23 | 1.14 ± 1.41 |
F | – | 0.294 | 0.098 | 3.351 | 0.241 | 1.714 | 2.098 | 0.487 |
p | – | 0.588 | 0.755 | 0.068 | 0.619 | 0.191 | 0.148 | 0.486 |
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Wu, D.-F.; Yin, R.-X.; Cao, X.-L.; Chen, W.-X. Association between Single Nucleotide Polymorphism rs1044925 and the Risk of Coronary Artery Disease and Ischemic Stroke. Int. J. Mol. Sci. 2014, 15, 3546-3559. https://doi.org/10.3390/ijms15033546
Wu D-F, Yin R-X, Cao X-L, Chen W-X. Association between Single Nucleotide Polymorphism rs1044925 and the Risk of Coronary Artery Disease and Ischemic Stroke. International Journal of Molecular Sciences. 2014; 15(3):3546-3559. https://doi.org/10.3390/ijms15033546
Chicago/Turabian StyleWu, Dong-Feng, Rui-Xing Yin, Xiao-Li Cao, and Wu-Xian Chen. 2014. "Association between Single Nucleotide Polymorphism rs1044925 and the Risk of Coronary Artery Disease and Ischemic Stroke" International Journal of Molecular Sciences 15, no. 3: 3546-3559. https://doi.org/10.3390/ijms15033546