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Int. J. Mol. Sci. 2014, 15(2), 3272-3286; doi:10.3390/ijms15023272

Characterization of Bactrocera dorsalis Serine Proteases and Evidence for Their Indirect Role in Insecticide Tolerance

Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China
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Received: 20 December 2013 / Revised: 9 February 2014 / Accepted: 12 February 2014 / Published: 21 February 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

The oriental fruit fly Bactrocera dorsalis (Hendel) causes devastating losses to agricultural crops world-wide and is considered to be an economically important pest. Little is known about the digestive enzymes such as serine proteases (SPs) in B. dorsalis, which are important both for energy supply and mitigation of fitness cost associated with insecticide tolerance. In this study, we identified five SP genes in the midgut of B. dorsalis, and the alignments of their deduced amino acid sequences revealed the presence of motifs conserved in the SP superfamily. Phylogenetic analyses with known SPs from other insect species suggested that three of them were trypsin-like proteases. Analyses of the expression profiles among the different developmental stages showed that all five genes were most abundant in larvae than in other stages. When larvae were continuously fed on diet containing 0.33 μg/g β-Cypermethrin, expression of all five genes were upregulated in the midgut but the larval development was delayed. Biochemical assays were consistent with the increased protease activity exhibited by SPs in the midgut after treatment with β-Cypermethrin. Taken together, these findings provide evidence for the hypothesis that enhanced SP activity may play an indirect role in relieving the toxicity stress of insecticide in B. dorsalis.
Keywords: Bactrocera dorsalis; serine protease; β-Cypermethrin; protease activity; expression profiles Bactrocera dorsalis; serine protease; β-Cypermethrin; protease activity; expression profiles
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Hou, M.-Z.; Shen, G.-M.; Wei, D.; Li, Y.-L.; Dou, W.; Wang, J.-J. Characterization of Bactrocera dorsalis Serine Proteases and Evidence for Their Indirect Role in Insecticide Tolerance. Int. J. Mol. Sci. 2014, 15, 3272-3286.

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