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Int. J. Mol. Sci. 2014, 15(2), 2826-2839; doi:10.3390/ijms15022826

The Anticoagulant Effect of PGI2S and tPA in Transgenic Umbilical Vein Endothelial Cells Is Linked to Up-Regulation of PKA and PKC

1
Cardiovascular Surgery Department, Second Peopleʼs Hospital of Guangdong Province, 1 Shi-liu Gang Road, Guangzhou 510317, China
2
State Key Laboratory of Oncology in Southern China, Sun Yat-sen University Cancer Center, 651 Dongfeng Road East, Guangzhou 510060, China
3
Department of Gynecology, Sun Yat-sen University Cancer Center, 651 Dongfeng Road East, Guangzhou 510060, China
4
Department of Chest, Sun Yat-sen University Cancer Center, 651 Dongfeng Road East, Guangzhou 510060, China
5
Department of Chest, Second People's Hospital of Guangdong Province, 1 Shi-liu Gang Road, Guangzhou 510317, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 23 November 2013 / Revised: 10 February 2014 / Accepted: 12 February 2014 / Published: 19 February 2014
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Abstract

The selection of vascular grafts for coronary artery bypass surgery is crucial for a positive outcome. This study aimed to establish a novel line of vascular endothelial cells with a potent anticoagulant effect. A lentiviral vector was used to stably transfect human umbilical vein endothelial cells (HUVECs) with PGI2S alone (HUVEC-PGI2S) or both PGI2S and tPA (HUVEC-PGI2S-tPA). Both HUVEC-PGI2S and HUVEC-PGI2S-tPA cells over-expressing PGI2S and tPA were compared to mock-transfected cells. The enzyme-linked immuno sorbent assay (ELISAs) demonstrated that the anticoagulation components, ATIII and PLG, were up-regulated and coagulation factor FVIII was down-regulated in both cell lines. QRT-PCR and western blotting demonstrated the vasodilation and platelet disaggregation proteins PKA, PKC, and PTGIR were up-regulated in both cell lines, but MAPK expression was not altered in either cell line. However, cell viability and colony formation assays and cell cycle analysis demonstrated that both cell lines had a lower rate of cell growth and induced G1 phase arrest. HUVEC-PGI2S and HUVEC-PGI2S-tPA cells have a potent anticoagulant effect and their use in vascular heterografts may decrease the risk of thrombosis. View Full-Text
Keywords: anticoagulant; coronary artery bypass graft; endothelial cells; PGI2S; tPA anticoagulant; coronary artery bypass graft; endothelial cells; PGI2S; tPA
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Wang, J.-H.; Yuan, L.-J.; Zhong, Z.-M.; Wen, Z.-S.; Deng, J.-M.; Liang, R.-X.; Zheng, M. The Anticoagulant Effect of PGI2S and tPA in Transgenic Umbilical Vein Endothelial Cells Is Linked to Up-Regulation of PKA and PKC. Int. J. Mol. Sci. 2014, 15, 2826-2839.

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