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Int. J. Mol. Sci. 2014, 15(2), 2738-2760; doi:10.3390/ijms15022738
Article

ScChi, Encoding an Acidic Class III Chitinase of Sugarcane, Confers Positive Responses to Biotic and Abiotic Stresses in Sugarcane

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Received: 13 January 2014; in revised form: 27 January 2014 / Accepted: 10 February 2014 / Published: 18 February 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: Chitinases (EC 3.2.2.14), expressed during the plant-pathogen interaction, are associated with plant defense against pathogens. In the present study, a positive correlation between chitinase activity and sugarcane smut resistance was found. ScChi (GenBank accession no. KF664180), a Class III chitinase gene, encoded a 31.37 kDa polypeptide, was cloned and identified. Subcellular localization revealed ScChi targeting to the nucleus, cytoplasm and the plasma membrane. Real-time quantitative PCR (RT-qPCR) results showed that ScChi was highly expressed in leaf and stem epidermal tissues. The ScChi transcript was both higher and maintained longer in the resistance cultivar during challenge with Sporisorium scitamineum. The ScChi also showed an obvious induction of transcription after treatment with SA (salicylic acid), H2O2, MeJA (methyl jasmonate), ABA (abscisic acid), NaCl, CuCl2, PEG (polyethylene glycol) and low temperature (4 °C). The expression levels of ScChi and six immunity associated marker genes were upregulated by the transient overexpression of ScChi. Besides, histochemical assay of Nicotiana benthamiana leaves overexpressing pCAMBIA 1301-ScChi exhibited deep DAB (3,3'-diaminobenzidinesolution) staining color and high conductivity, indicating the high level of H2O2 accumulation. These results suggest a close relationship between the expression of ScChi and plant immunity. In conclusion, the positive responses of ScChi to the biotic and abiotic stimuli reveal that this gene is a stress-related gene of sugarcane.
Keywords: chitinase; Saccharum officinarum; Sporisorium scitamineum; subcellular localization; gene expression; abiotic stress chitinase; Saccharum officinarum; Sporisorium scitamineum; subcellular localization; gene expression; abiotic stress
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Su, Y.; Xu, L.; Fu, Z.; Yang, Y.; Guo, J.; Wang, S.; Que, Y. ScChi, Encoding an Acidic Class III Chitinase of Sugarcane, Confers Positive Responses to Biotic and Abiotic Stresses in Sugarcane. Int. J. Mol. Sci. 2014, 15, 2738-2760.

AMA Style

Su Y, Xu L, Fu Z, Yang Y, Guo J, Wang S, Que Y. ScChi, Encoding an Acidic Class III Chitinase of Sugarcane, Confers Positive Responses to Biotic and Abiotic Stresses in Sugarcane. International Journal of Molecular Sciences. 2014; 15(2):2738-2760.

Chicago/Turabian Style

Su, Yachun; Xu, Liping; Fu, Zhiwei; Yang, Yuting; Guo, Jinlong; Wang, Shanshan; Que, Youxiong. 2014. "ScChi, Encoding an Acidic Class III Chitinase of Sugarcane, Confers Positive Responses to Biotic and Abiotic Stresses in Sugarcane." Int. J. Mol. Sci. 15, no. 2: 2738-2760.



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