Effect of Glucans from Caripia montagnei Mushroom on TNBS-Induced Colitis
Abstract
:1. Introduction
2. Results
2.1. Chemical Analysis
2.2. FT-IR Spectrum
2.3. NMR
2.4. Macroscopic Analysis
2.5. Activity of Myeloperoxidase (MPO)
2.6. Activity of Alkaline Phosphatase
2.7. Nitric Oxide
2.8. Activity of Catalase
2.9. Cytokine Analysis
2.10. Histological Analyses
3. Discussion
4. Experimental Section
4.1. Mushrooms
4.2. Animals
4.3. Materials
4.4. Polysaccharide Extraction and Fractionation
4.5. Chemical Analysis
4.6. Infrared Analysis
4.7. Nuclear Magnetic Resonance (NMR)
4.8. Anti-Inflammatory Activity in the Ulcerative Colitis Model Induced by 2,4,6-Trinitrobenzene Sulfonic Acid (TNBS)
4.9. Treatment of Colitis with Polysaccharides
4.10. Macroscopic Analysis
- Score 0: No injuries.
- Score 1: Hyperemia without ulceration.
- Score 2: Linear ulceration without inflammation.
- Score 3: Linear ulceration with inflammation.
- Score 4: Two or more ulcerations and inflammation.
- Score 5: Two or more ulcerations and inflammation or ulceration longer than 1 cm along the colon.
- Score 6–10: If the lesions are greater than 2 cm in length longitudinally, 1 point for every extra inch will be awarded.
4.11. Assessment of Myeloperoxidase Activity
4.12. Evaluation of Alkaline Phosphatase Activity
4.13. Nitric Oxide
4.14. Evaluation of Catalase Activity
4.15. Analysis of Cytokines
4.16. Histological Analyses
4.17. Statistical Analyses
5. Conclusions
Acknowledgments
Conflicts of Interest
References
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Da S. Nascimento Santos, M.; De M. Magalhães, J.E.; Castro, L.S.E.P.W.; De Sousa Pinheiro, T.; Sabry, D.A.; Nobre, L.T.D.B.; Lima, J.P.M.S.; Baseia, I.G.; Leite, E.L. Effect of Glucans from Caripia montagnei Mushroom on TNBS-Induced Colitis. Int. J. Mol. Sci. 2014, 15, 2368-2385. https://doi.org/10.3390/ijms15022368
Da S. Nascimento Santos M, De M. Magalhães JE, Castro LSEPW, De Sousa Pinheiro T, Sabry DA, Nobre LTDB, Lima JPMS, Baseia IG, Leite EL. Effect of Glucans from Caripia montagnei Mushroom on TNBS-Induced Colitis. International Journal of Molecular Sciences. 2014; 15(2):2368-2385. https://doi.org/10.3390/ijms15022368
Chicago/Turabian StyleDa S. Nascimento Santos, Marilia, Joedyson Emmanuel De M. Magalhães, Luiza Sheyla Evenni P. Will Castro, Thuane De Sousa Pinheiro, Diego Araujo Sabry, Leonardo Thiago Duarte B. Nobre, João Paulo Matos Santos Lima, Iuri Goulart Baseia, and Edda Lisboa Leite. 2014. "Effect of Glucans from Caripia montagnei Mushroom on TNBS-Induced Colitis" International Journal of Molecular Sciences 15, no. 2: 2368-2385. https://doi.org/10.3390/ijms15022368