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Int. J. Mol. Sci. 2014, 15(2), 2075-2086; doi:10.3390/ijms15022075
Article

Cellular Behavior of Human Adipose-Derived Stem Cells on Wettable Gradient Polyethylene Surfaces

1
, 1
, 2
 and 2,*
Received: 22 November 2013; in revised form: 21 January 2014 / Accepted: 22 January 2014 / Published: 28 January 2014
(This article belongs to the Special Issue Interaction between Nano-Structure Materials and Cells)
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Abstract: Appropriate surface wettability and roughness of biomaterials is an important factor in cell attachment and proliferation. In this study, we investigated the correlation between surface wettability and roughness, and biological response in human adipose-derived stem cells (hADSCs). We prepared wettable and rough gradient polyethylene (PE) surfaces by increasing the power of a radio frequency corona discharge apparatus with knife-type electrodes over a moving sample bed. The PE changed gradually from hydrophobic and smooth surfaces to hydrophilic (water contact angle, 90° to ~50°) and rough (80 to ~120 nm) surfaces as the power increased. We found that hADSCs adhered better to highly hydrophilic and rough surfaces and showed broadly stretched morphology compared with that on hydrophobic and smooth surfaces. The proliferation of hADSCs on hydrophilic and rough surfaces was also higher than that on hydrophobic and smooth surfaces. Furthermore, integrin beta 1 gene expression, an indicator of attachment, and heat shock protein 70 gene expression were high on hydrophobic and smooth surfaces. These results indicate that the cellular behavior of hADSCs on gradient surface depends on surface properties, wettability and roughness.
Keywords: surface wettability; roughness; corona discharge; human adipose derived stem cells; integrin beta 1 gene; heat shock protein surface wettability; roughness; corona discharge; human adipose derived stem cells; integrin beta 1 gene; heat shock protein
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Ahn, H.H.; Lee, I.W.; Lee, H.B.; Kim, M.S. Cellular Behavior of Human Adipose-Derived Stem Cells on Wettable Gradient Polyethylene Surfaces. Int. J. Mol. Sci. 2014, 15, 2075-2086.

AMA Style

Ahn HH, Lee IW, Lee HB, Kim MS. Cellular Behavior of Human Adipose-Derived Stem Cells on Wettable Gradient Polyethylene Surfaces. International Journal of Molecular Sciences. 2014; 15(2):2075-2086.

Chicago/Turabian Style

Ahn, Hyun H.; Lee, Il W.; Lee, Hai B.; Kim, Moon S. 2014. "Cellular Behavior of Human Adipose-Derived Stem Cells on Wettable Gradient Polyethylene Surfaces." Int. J. Mol. Sci. 15, no. 2: 2075-2086.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert