Int. J. Mol. Sci. 2014, 15(2), 1915-1926; doi:10.3390/ijms15021915
Article

β-Conglycinin Reduces the Tight Junction Occludin and ZO-1 Expression in IPEC-J2

1,2email, 1,2,* email, 1email, 1email, 3email and 1email
Received: 24 November 2013; in revised form: 12 January 2014 / Accepted: 20 January 2014 / Published: 27 January 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Soybean allergy presents a health threat to humans and animals. The mechanism by which food/feed allergen β-conglycinin injures the intestinal barrier has not been well understood. In this study, the changes of epithelial permeability, integrity, metabolic activity, the tight junction (TJ) distribution and expression induced by β-conglycinin were evaluated using IPEC-J2 model. The results showed a significant decrease of trans-epithelial electrical resistance (TEER) (p < 0.001) and metabolic activity (p < 0.001) and a remarkable increase of alkaline phosphatase (AP) activity (p < 0.001) in a dose-dependent manner. The expression levels of tight junction occludin and ZO-1 were decreased (p < 0.05). The reduced fluorescence of targets and change of cellular morphology were recorded. The tight junction occludin and ZO-1 mRNA expression linearly declined with increasing β-conglycinin (p < 0.001).
Keywords: β-conglycinin; intestinal barrier; tight junction; food allergen; IPEC-J2
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MDPI and ACS Style

Zhao, Y.; Qin, G.; Han, R.; Wang, J.; Zhang, X.; Liu, D. β-Conglycinin Reduces the Tight Junction Occludin and ZO-1 Expression in IPEC-J2. Int. J. Mol. Sci. 2014, 15, 1915-1926.

AMA Style

Zhao Y, Qin G, Han R, Wang J, Zhang X, Liu D. β-Conglycinin Reduces the Tight Junction Occludin and ZO-1 Expression in IPEC-J2. International Journal of Molecular Sciences. 2014; 15(2):1915-1926.

Chicago/Turabian Style

Zhao, Yuan; Qin, Guixin; Han, Rui; Wang, Jun; Zhang, Xiaodong; Liu, Dandan. 2014. "β-Conglycinin Reduces the Tight Junction Occludin and ZO-1 Expression in IPEC-J2." Int. J. Mol. Sci. 15, no. 2: 1915-1926.

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