Abstract: Soybean allergy presents a health threat to humans and animals. The mechanism by which food/feed allergen β-conglycinin injures the intestinal barrier has not been well understood. In this study, the changes of epithelial permeability, integrity, metabolic activity, the tight junction (TJ) distribution and expression induced by β-conglycinin were evaluated using IPEC-J2 model. The results showed a significant decrease of trans-epithelial electrical resistance (TEER) (p < 0.001) and metabolic activity (p < 0.001) and a remarkable increase of alkaline phosphatase (AP) activity (p < 0.001) in a dose-dependent manner. The expression levels of tight junction occludin and ZO-1 were decreased (p < 0.05). The reduced fluorescence of targets and change of cellular morphology were recorded. The tight junction occludin and ZO-1 mRNA expression linearly declined with increasing β-conglycinin (p < 0.001).
Keywords: β-conglycinin; intestinal barrier; tight junction; food allergen; IPEC-J2
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Zhao, Y.; Qin, G.; Han, R.; Wang, J.; Zhang, X.; Liu, D. β-Conglycinin Reduces the Tight Junction Occludin and ZO-1 Expression in IPEC-J2. Int. J. Mol. Sci. 2014, 15, 1915-1926.
Zhao Y, Qin G, Han R, Wang J, Zhang X, Liu D. β-Conglycinin Reduces the Tight Junction Occludin and ZO-1 Expression in IPEC-J2. International Journal of Molecular Sciences. 2014; 15(2):1915-1926.
Zhao, Yuan; Qin, Guixin; Han, Rui; Wang, Jun; Zhang, Xiaodong; Liu, Dandan. 2014. "β-Conglycinin Reduces the Tight Junction Occludin and ZO-1 Expression in IPEC-J2." Int. J. Mol. Sci. 15, no. 2: 1915-1926.