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International Journal of Molecular Science Best Paper Award 2014
Int. J. Mol. Sci. 2014, 15(2), 1686-1699; doi:10.3390/ijms15021686
Article

Chronic Exposure to Rhodobacter Sphaeroides Extract Lycogen™ Prevents UVA-Induced Malondialdehyde Accumulation and Procollagen I Down-Regulation in Human Dermal Fibroblasts

1,†
,
2,†
,
1
,
3,4
,
1
 and
5,*
1 Department of Health and Nutrition, Chia-Nan University of Pharmacy and Science, Tainan 71710, Taiwan 2 Department of Medical Research and Education, Taipei Veterans General Hospital, Taipei 11217, Taiwan 3 Asia-Pacific Biotech Developing, Inc., Kaohsiung 80681, Taiwan 4 Department of Marine Biotechnology and Resources, National Sun Yat-Sen University, Kaohsiung 80424, Taiwan 5 Department and Institute of Cosmetic Science, Chia-Nan University of Pharmacy and Science, Tainan 71710, Taiwan These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 31 October 2013 / Revised: 27 December 2013 / Accepted: 10 January 2014 / Published: 23 January 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

UVA contributes to the pathogenesis of skin aging by downregulation of procollagen I content and induction of matrix metalloproteinase (MMP)-associated responses. Application of antioxidants such as lycopene has been demonstrated as a convenient way to achieve protection against skin aging. Lycogen™, derived from the extracts of Rhodobacter sphaeroides, exerts several biological effects similar to that of lycopene whereas most of its anti-aging efficacy remains uncertain. In this study, we attempted to examine whether Lycogen™ could suppress malondialdehyde (MDA) accumulation and restore downregulated procollagen I expression induced by UVA exposure. In human dermal fibroblasts Hs68 cells, UVA repressed cell viability and decreased procollagen I protein content accompanied with the induction of MMP-1 and MDA accumulation. Remarkably, incubation with 50 µM Lycogen™ for 24 h ameliorated UVA-induced cell death and restored UVA-induced downregulation of procollagen in a dose-related manner. Lycogen™ treatment also prevented the UVA-induced MMP-1 upregulation and intracellular MDA generation in Hs68 cells. Activation of NFκB levels, one of the downstream events induced by UVA irradiation and MMP-1 induction, were also prevented by Lycogen™ administration. Taken together, our findings demonstrate that Lycogen™ may be an alternative agent that prevents UVA-induced skin aging and could be used in cosmetic and pharmaceutical applications.
Keywords: Lycogen™; skin aging; UVA; procollagen I; matrix metalloproteinase Lycogen™; skin aging; UVA; procollagen I; matrix metalloproteinase
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Yang, T.-H.; Lai, Y.-H.; Lin, T.-P.; Liu, W.-S.; Kuan, L.-C.; Liu, C.-C. Chronic Exposure to Rhodobacter Sphaeroides Extract Lycogen™ Prevents UVA-Induced Malondialdehyde Accumulation and Procollagen I Down-Regulation in Human Dermal Fibroblasts. Int. J. Mol. Sci. 2014, 15, 1686-1699.

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