Isolation and Structural Elucidation of Antiproliferative Compounds of Lipidic Fractions from White Shrimp Muscle (Litopenaeus vannamei)
Abstract
:1. Introduction
2. Results and Discussion
2.1. Lipidic Extraction and Partition
Antiproliferative Activity of Fractions Obtained by Partition
Fraction | 400 µg/mL | 200 µg/mL | 100 µg/mL |
---|---|---|---|
Hexanic | 80.69 ± 4.62 a | 86.78 ± 10.13 a | 96.59 ± 6.94 a |
Methanolic | 23.29 ± 5.61 b | 33.61 ± 13.8 b | 90.44 ± 8.27 a |
2.2. Analysis of Lipidic Composition by RP-HPLC
Antiproliferative Activity of Methanolic Sub-Fractions Obtained by Semi-Preparative RP-HPLC
2.3. Fractioning by Open Column Chromatography
2.3.1. Chemical/Structural Characterization of M12 Fractions
Fraction | 200 µg/mL | 100 µg/mL |
---|---|---|
M1 | 129.22 ±16.75 a | 113.60 ± 13.22 b |
M2 | 133.53 ± 11.62 a | 118.22 ± 8.77 b |
M3 | 129.76 ± 0.51 a | 120.17 ± 10.67 b |
M4 | 98.06 ± 3.60 b | 117.86 ± 9.05 b |
M5 | 2.004 ± 2.05 e | 74.61 ± 5.92 c,d |
M6 | 3.402 ± 3.31 e | 69.14 ± 10.94 c,d |
M7 | 57.72 ± 6.02 c,d | 171.56 ± 8.20 a |
M8 | 6.379 ± 4.48 e | 16.16 ± 4.19 e,f |
M9 | 2.19 ± 0.63 e | 3.28 ± 1.26 f |
M10 | 6.20 ± 1.48 e | 16.76 ± 4.55 f |
M11 | 13.00 ± 8.505 e | 44.83 ± 12.21 d,e |
M12 | 2.67 ± 0.92 e | 2.79 ± 2.84 f |
M13 | 8.51 ± 3.79 e | 22.84 ± 2.59 e,f |
M14 | 67.63 ± 8.75 d | 105.95 ± 13.78 b,c |
M15 | 97.93 ± 5.67 b | 136.19 ± 2.69 a,b |
2.3.2. Antiproliferative Activity of M12 Fractions
3. Experimental Section
3.1. Testing Species
3.2. Analysis of Lipidic Composition by RP-HPLC
3.3. Isolation of Bioactive Component by Column Chromatography
3.4. Cell Lines
3.5. Antiproliferation Assay
3.6. 1H NMR Analysis
3.7. GC-MS Analysis
3.8. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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López-Saiz, C.-M.; Velázquez, C.; Hernández, J.; Cinco-Moroyoqui, F.-J.; Plascencia-Jatomea, M.; Robles-Sánchez, M.; Machi-Lara, L.; Burgos-Hernández, A. Isolation and Structural Elucidation of Antiproliferative Compounds of Lipidic Fractions from White Shrimp Muscle (Litopenaeus vannamei). Int. J. Mol. Sci. 2014, 15, 23555-23570. https://doi.org/10.3390/ijms151223555
López-Saiz C-M, Velázquez C, Hernández J, Cinco-Moroyoqui F-J, Plascencia-Jatomea M, Robles-Sánchez M, Machi-Lara L, Burgos-Hernández A. Isolation and Structural Elucidation of Antiproliferative Compounds of Lipidic Fractions from White Shrimp Muscle (Litopenaeus vannamei). International Journal of Molecular Sciences. 2014; 15(12):23555-23570. https://doi.org/10.3390/ijms151223555
Chicago/Turabian StyleLópez-Saiz, Carmen-María, Carlos Velázquez, Javier Hernández, Francisco-Javier Cinco-Moroyoqui, Maribel Plascencia-Jatomea, Maribel Robles-Sánchez, Lorena Machi-Lara, and Armando Burgos-Hernández. 2014. "Isolation and Structural Elucidation of Antiproliferative Compounds of Lipidic Fractions from White Shrimp Muscle (Litopenaeus vannamei)" International Journal of Molecular Sciences 15, no. 12: 23555-23570. https://doi.org/10.3390/ijms151223555