Effect of Hydrogen Peroxide on the Biosynthesis of Heme and Proteins: Potential Implications for the Partitioning of Glu-tRNAGlu between These Pathways
Abstract
:1. Introduction
2. Results and Discussion
2.1. Hydrogen Peroxide Inactivates Glutamyl-tRNA Synthetase 1 (GluRS1) and Reduces Heme Levels in A. ferrooxidans
2.2. Glutamyl-tRNA Reductase (GluTR) from A. ferrooxidans Is Inactivated by Hydrogen Peroxide
Type of GluTR (H2O2) | GluTR (1/4) 250 µM H2O2 | GluTR (1/4) 500 µM H2O2 | GluTR (1/12) 500 µM H2O2 |
---|---|---|---|
kobs (s−1) | 3.18 × 10−4 ± (4.43 × 10−5) | 6.96 × 10−4 ± (4.49 × 10−5) | 4.82 × 10−4 ± (2.29 × 10−5) |
2.3. Hydrogen Peroxide Decreases the Stimulation of GluTR by Glutamate Semialdehyde 1-2 Aminomutase (GSAM)
2.4. Elongation Factor Tu (EF-Tu) Is not Affected by Treatment with H2O2
2.5. Competition of GluTR and EF-Tu for Glu-tRNAGlu Is Affected by H2O2
2.6. Discussion
3. Experimental Section
3.1. Bacterial Strains and Culture Conditions
3.2. Overproduction and Purification of Proteins
3.3. Preparation and Purification of tRNA Transcripts
3.4. Preparation of A. ferrooxidans Cellular Extracts
3.5. GluRS Activity
3.6. GluTR Activity
3.7. Binding of Glutamyl-tRNAGlu to EF-Tu
3.8. Protein Oxidation
3.9. Heme Measurement
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Farah, C.; Levicán, G.; Ibba, M.; Orellana, O. Effect of Hydrogen Peroxide on the Biosynthesis of Heme and Proteins: Potential Implications for the Partitioning of Glu-tRNAGlu between These Pathways. Int. J. Mol. Sci. 2014, 15, 23011-23023. https://doi.org/10.3390/ijms151223011
Farah C, Levicán G, Ibba M, Orellana O. Effect of Hydrogen Peroxide on the Biosynthesis of Heme and Proteins: Potential Implications for the Partitioning of Glu-tRNAGlu between These Pathways. International Journal of Molecular Sciences. 2014; 15(12):23011-23023. https://doi.org/10.3390/ijms151223011
Chicago/Turabian StyleFarah, Carolina, Gloria Levicán, Michael Ibba, and Omar Orellana. 2014. "Effect of Hydrogen Peroxide on the Biosynthesis of Heme and Proteins: Potential Implications for the Partitioning of Glu-tRNAGlu between These Pathways" International Journal of Molecular Sciences 15, no. 12: 23011-23023. https://doi.org/10.3390/ijms151223011