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Int. J. Mol. Sci. 2014, 15(12), 22678-22693; doi:10.3390/ijms151222678

Sll0528, a Site-2-Protease, Is Critically Involved in Cold, Salt and Hyperosmotic Stress Acclimation of Cyanobacterium Synechocystis sp. PCC 6803

College of Light Industry and Food Sciences, South China University of Technology, 381 Wushan Road, Guangzhou 510641, China
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Received: 30 September 2014 / Revised: 21 November 2014 / Accepted: 25 November 2014 / Published: 8 December 2014
(This article belongs to the Special Issue Signalling Molecules and Signal Transduction in Cells 2014)
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Abstract

Site-2-proteases (S2Ps) mediated proteolysis of transmembrane transcriptional regulators is a conserved mechanism to regulate transmembrane signaling. The universal presence of S2P homologs in different cyanobacterial genomes suggest conserved and fundamental functions, though limited data has been available. Here we provide the first evidence that Sll0528, a site-2-protease in Synechocystis sp. PCC 6803 is crucial for salt, cold and hyperosmotic stress acclimation. Remarkable induction of sll0528 gene expression was observed under salt, cold and hyperosmotic stress, much higher than induction of the other three S2Ps. Knock-out of sll0528 gene in wild type Synechocystis sp. PCC 6803 increased their sensitivity to salt, cold and hyperosmotic stress, as revealed by retarded growth, reduced pigments and disrupted photosystems. The sll0528 gene was induced to a much smaller extent by high light and mixotrophic growth with glucose. Similar growth responses of the sll0528 knockout mutant and wild type under high light and mixotrophic growth indicated that sll0528 was dispensable for these conditions. Recombinant Sll0528 protein could cleave beta-casein into smaller fragments. These results together suggest that the Sll0528 metalloprotease plays a role in the stress response and lays the foundation for further investigation of its mechanism, as well as providing hints for the functional analysis of other S2Ps in cyanobacteria. View Full-Text
Keywords: Synechocystis sp. PCC 6803; site-2-proteases; Sll0528; cold stress; salt stress; hyperosmotic stress; RT-qPCR; knock-out mutant; in vitro protease activity Synechocystis sp. PCC 6803; site-2-proteases; Sll0528; cold stress; salt stress; hyperosmotic stress; RT-qPCR; knock-out mutant; in vitro protease activity
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Lei, H.; Chen, G.; Wang, Y.; Ding, Q.; Wei, D. Sll0528, a Site-2-Protease, Is Critically Involved in Cold, Salt and Hyperosmotic Stress Acclimation of Cyanobacterium Synechocystis sp. PCC 6803. Int. J. Mol. Sci. 2014, 15, 22678-22693.

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