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Int. J. Mol. Sci. 2014, 15(11), 21286-21298; doi:10.3390/ijms151121286

Enhanced Levels of Interleukin-8 Are Associated with Hepatitis B Virus Infection and Resistance to Interferon-Alpha Therapy

Department of Laboratory Medicine, Second Affiliated Hospital of Anhui Medical University, Hefei 230601, China
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Received: 21 July 2014 / Revised: 6 November 2014 / Accepted: 11 November 2014 / Published: 17 November 2014
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
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Abstract

The objective of this study was to analyze the expression levels of IL-8 in serum and liver tissues from patients with chronic hepatitis B (CHB) infection and to investigate whether IL-8 may antagonize interferon-alpha (IFN-α) antiviral activity against HBV. IL-8 expression in serum was determined by enzyme linked immunosorbent assay (ELISA), and fluorescence-based quantitative real-time PCR (RT-qPCR) was used to measure IL-8 mRNA in peripheral blood mononuclear cells (PBMCs) in patients with CHB. IL-8 protein expression was detected in liver biopsy tissues by immunohistochemistry. In addition, the differences in serum IL-8 and PBMCs mRNA levels were also observed in patients with different anti-viral responses to IFN-α. Compared to normal controls, serum IL-8 protein and mRNA levels were increased in CHB patients, IL-8 levels were positively correlated with the severity of liver inflammation/fibrosis. Moreover, serum IL-8 protein and mRNA levels were positively correlated with serum alanine aminotransferase (ALT) level and negatively correlated with serum prealbumin (PA) level. IL-8 expression was mainly located in portal area of liver tissues and was increased with the severity of liver inflammation and fibrosis stage. The expression serum and mRNA levels of IL-8 in the CHB patients with a complete response to IFN-α are significantly lower than that of the patients with non-response to IFN-α treatment. It is suggested that IL-8 might play important roles in the pathogenesis of CHB. Moreover, interferon resistance may be related to the up-regulation of IL-8 expression in the patients did not respond to IFN-α treatment. View Full-Text
Keywords: interleukin-8; interferon-alpha; chronic hepatitis B; inflammation; fibrosis interleukin-8; interferon-alpha; chronic hepatitis B; inflammation; fibrosis
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MDPI and ACS Style

Yang, K.; Guan, S.-H.; Zhang, H.; Pan, Y.; Wu, Y.-Y.; Wang, A.-H.; Sun, B.-B. Enhanced Levels of Interleukin-8 Are Associated with Hepatitis B Virus Infection and Resistance to Interferon-Alpha Therapy. Int. J. Mol. Sci. 2014, 15, 21286-21298.

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