Next Article in Journal
Berteroin Present in Cruciferous Vegetables Exerts Potent Anti-Inflammatory Properties in Murine Macrophages and Mouse Skin
Next Article in Special Issue
A Review of Polyphenolics in Oak Woods
Previous Article in Journal
Potential Roles of BMP9 in Liver Fibrosis
Previous Article in Special Issue
Rosmarinus Officinalis Leaves as a Natural Source of Bioactive Compounds
Article Menu
Issue 11 (November) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2014, 15(11), 20668-20685; doi:10.3390/ijms151120668

Characterization of Flavonoid Glycosides from Fenugreek (Trigonella foenum-graecum) Crude Seeds by HPLC–DAD–ESI/MS Analysis

1
Institute of Food science, Technology and Nutrition of the Spanish National Research Council (ICTAN–CSIC), Juan de la Cierva 3, Madrid 28006, Spain
2
Team of Organic Chemistry and Physico-Chemical Studies, Ecole Normale Superieure, Mohammed V University of Rabat, P.O. Box 5118, 10200 Rabat, Morocco
*
Authors to whom correspondence should be addressed.
Received: 27 September 2014 / Revised: 28 October 2014 / Accepted: 3 November 2014 / Published: 11 November 2014
(This article belongs to the Special Issue Bioactive Phenolics and Polyphenols)
View Full-Text   |   Download PDF [689 KB, uploaded 11 November 2014]   |  

Abstract

Fenugreek (Trigonella foenum-graecum) is a medicinal plant which is widely used for its pharmacological properties. In this study the phenolic composition of fenugreek crude seeds originating from Morocco has been investigated. Extraction was performed from defatted seeds by a hydromethanolic solution using an Accelerated Solvent Extractor. HPLC technique coupled to negative ion electrospray ionization mass spectrometry and diode array detection was employed to identify the polyphenol in the obtained extract. The obtained results allowed the detection of 32 phenolic compounds among which various flavonoid glycosides and phenolic acids have been tentatively identified on the basis of their UV and MS spectra, and comparisons with standards when available, as well as with literature data. A systematic study of the obtained MS spectra and the observed fragmentation showed that most of the identified compounds were acylated and non-acylated flavonoids with apigenin, luteolin and kaempferol as aglycons. Hydroxycinnamic acids mostly dominated by caffeic acid derivatives were also detected. The quantitative analysis of the identified compounds showed that the phenolic composition of the studied crude fenugreek seeds was predominantly acylated and non-acylated flavone derivatives with apigenin as the main aglycon. View Full-Text
Keywords: Trigonella foenum-graecum; fenugreek seeds; Accelerated Solvent Extractor (ASE); flavonoids; flavones; flavonols; acylated glycosides; hydrocinnamic acids; mass spectrometry; LC–MS analysis Trigonella foenum-graecum; fenugreek seeds; Accelerated Solvent Extractor (ASE); flavonoids; flavones; flavonols; acylated glycosides; hydrocinnamic acids; mass spectrometry; LC–MS analysis
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Benayad, Z.; Gómez-Cordovés, C.; Es-Safi, N.E. Characterization of Flavonoid Glycosides from Fenugreek (Trigonella foenum-graecum) Crude Seeds by HPLC–DAD–ESI/MS Analysis. Int. J. Mol. Sci. 2014, 15, 20668-20685.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top