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Int. J. Mol. Sci. 2014, 15(11), 19472-19486; doi:10.3390/ijms151119472

Expression Analysis of Immune Related Genes Identified from the Coelomocytes of Sea Cucumber (Apostichopus japonicus) in Response to LPS Challenge

Liaoning Key Lab of Marine Fishery Molecular Biology, Liaoning Ocean and Fisheries Science Research Institute, Dalian 116023, China
These authors contributed equally to this work.
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Received: 30 May 2014 / Revised: 29 September 2014 / Accepted: 14 October 2014 / Published: 27 October 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

The sea cucumber (Apostichopus japonicus) occupies a basal position during the evolution of deuterostomes and is also an important aquaculture species. In order to identify more immune effectors, transcriptome sequencing of A. japonicus coelomocytes in response to lipopolysaccharide (LPS) challenge was performed using the Illumina HiSeq™ 2000 platform. One hundred and seven differentially expressed genes were selected and divided into four functional categories including pathogen recognition (25 genes), reorganization of cytoskeleton (27 genes), inflammation (41 genes) and apoptosis (14 genes). They were analyzed to elucidate the mechanisms of host-pathogen interactions and downstream signaling transduction. Quantitative real-time polymerase chain reactions (qRT-PCRs) of 10 representative genes validated the accuracy and reliability of RNA sequencing results with the correlation coefficients from 0.88 to 0.98 and p-value <0.05. Expression analysis of immune-related genes after LPS challenge will be useful in understanding the immune response mechanisms of A. japonicus against pathogen invasion and developing strategies for resistant markers selection. View Full-Text
Keywords: sea cucumber (Apostichopus japonicus); differentially expressed genes; quantitative real-time polymerase chain reaction sea cucumber (Apostichopus japonicus); differentially expressed genes; quantitative real-time polymerase chain reaction
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Dong, Y.; Sun, H.; Zhou, Z.; Yang, A.; Chen, Z.; Guan, X.; Gao, S.; Wang, B.; Jiang, B.; Jiang, J. Expression Analysis of Immune Related Genes Identified from the Coelomocytes of Sea Cucumber (Apostichopus japonicus) in Response to LPS Challenge. Int. J. Mol. Sci. 2014, 15, 19472-19486.

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