Intermittently Administered Parathyroid Hormone [1–34] Promotes Tendon-Bone Healing in a Rat Model
Abstract
:1. Introduction
2. Results and Discussion
2.1. Results
2.1.1. Serum Chemistry
Concentration | Control | PTH | p-Value |
---|---|---|---|
Calcium (mmol/L) | 2.026 ± 0.396 | 2.383 ± 0.074 * | 0.012 |
AP (U/L) | 180.6 ± 44.7 | 228.5 ± 22.8 * | 0.007 |
Osteocalcin (pg/mL) | 353.07 ± 29.62 | 425.77 ± 15.98 * | 0.000 |
TRAP (mIU/mL) | 2.567 ± 0.892 | 2.938 ± 0.139 * | 0.000 |
2.1.2. Micro-CT
Items | Control | PTH | p-Value |
---|---|---|---|
Area (mm2) | 2.88 ± 0.32 | 2.35 ± 0.38 * | 0.043 |
BMD (mg/mL) | 0.3426 ± 0.0059 | 0.4009 ± 0.0083 * | 0.000 |
BV/TV (%) | 28.52 ± 1.56 | 33.43 ± 1.67 * | 0.001 |
BS/BV (1/mm) | 11.64225 ± 1.369840 | 16.24998 ± 1.580501 * | 0.001 |
BS/TV (1/mm) | 3.900066 ± 0.574287 | 4.625814 ± 0.652808 | 0.099 |
Tb.Th (mm) | 0.235796 ± 0.053191 | 0.349876 ± 0.071286 * | 0.021 |
Tb.N (1/mm) | 0.992012 ± 0.233659 | 1.243782 ± 0.260488 | 0.146 |
Tb.Sp (mm) | 1.061898 ± 0.099597 | 0.81308 ± 0.144448 * | 0.013 |
2.1.3. Biomechanical Test
Items | Control | PTH | p-Value |
---|---|---|---|
Failure load (N) | 8.17 ± 0.59 | 16.49 ± 4.90 * | 0.005 |
Stiffness (N/mm) | 2.48 ± 0.47 | 4.32 ± 1.95 | 0.102 |
2.1.4. Histological Staining
2.1.5. Immunohistochemistry Staining
2.2. Discussion
3. Experimental Section
3.1. Study Design
3.2. Surgical Procedure
3.3. Serum Chemistry
3.4. Micro-CT
3.5. Biomechanical Test
3.6. Histological and Immunohistochemical Analyses
3.7. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Bi, F.; Shi, Z.; Jiang, S.; Guo, P.; Yan, S. Intermittently Administered Parathyroid Hormone [1–34] Promotes Tendon-Bone Healing in a Rat Model. Int. J. Mol. Sci. 2014, 15, 17366-17379. https://doi.org/10.3390/ijms151017366
Bi F, Shi Z, Jiang S, Guo P, Yan S. Intermittently Administered Parathyroid Hormone [1–34] Promotes Tendon-Bone Healing in a Rat Model. International Journal of Molecular Sciences. 2014; 15(10):17366-17379. https://doi.org/10.3390/ijms151017366
Chicago/Turabian StyleBi, Fanggang, Zhongli Shi, Shuai Jiang, Peng Guo, and Shigui Yan. 2014. "Intermittently Administered Parathyroid Hormone [1–34] Promotes Tendon-Bone Healing in a Rat Model" International Journal of Molecular Sciences 15, no. 10: 17366-17379. https://doi.org/10.3390/ijms151017366