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Int. J. Mol. Sci. 2014, 15(1), 958-976; doi:10.3390/ijms15010958
Article

Genistein Induces Increase in Fluid pH, Na+ and HCO3 Concentration, SLC26A6 and SLC4A4 (NBCe1)-B Expression in the Uteri of Ovariectomized Rats

1,†
,
1,†
,
2
,
3
 and
1,†,*
1 Department of Physiology, Faculty of Medicine, University of Malaya, Lembah Pantai, Kuala Lumpur 50603, Malaysia 2 Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Lembah Pantai, Kuala Lumpur 50603, Malaysia 3 Department of Anatomy, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 24 November 2013 / Revised: 8 December 2013 / Accepted: 29 December 2013 / Published: 10 January 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Genistein has been reported to stimulate luminal HCO3 secretion. We hypothesized that genistein mediates this effect via SLC26A6 and SLC4A4 (NBCe1) transporters. Our study aimed to: investigate changes in uterine fluid pH, Na+ and HCO3 concentration and expression of uterine SLC26A6 and NBCe1 under genistein effect. Ovariectomized adult female rats received 25, 50 and 100 mg/kg/day genistein for a week with and without ICI 182780. A day after the last injection, in vivo uterine perfusion was performed to collect uterine fluid for Na+, HCO3 and pH determination. The animals were then sacrificed and uteri were removed for mRNA and protein expression analyses. SLC26A6 and NBCe1-A and NBCe1-B distribution were visualized by immunohistochemistry (IHC). Genistein at 50 and 100 mg/kg/day stimulates uterine fluid pH, Na+ and HCO3 concentration increase. Genistein at 100 mg/kg/day up-regulates the expression of SLC26A6 and SLC4A4 mRNA, which were reduced following concomitant ICI 182780 administration. In parallel, SLC26A6 and NBCe1-B protein expression were also increased following high dose genistein treatment and were localized mainly at the apical membrane of the luminal epithelia. SLC26A6 and NBCe1-B up-regulation by genistein could be responsible for the observed increase in the uterine fluid pH, Na+ and HCO3 concentration under this condition.
Keywords: genistein; SLC26A6; SLC4A4; uterine fluid genistein; SLC26A6; SLC4A4; uterine fluid
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Chinigarzadeh, A.; Kasim, N.F.; Muniandy, S.; Kassim, N.M.; Salleh, N. Genistein Induces Increase in Fluid pH, Na+ and HCO3 Concentration, SLC26A6 and SLC4A4 (NBCe1)-B Expression in the Uteri of Ovariectomized Rats. Int. J. Mol. Sci. 2014, 15, 958-976.

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