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Int. J. Mol. Sci. 2014, 15(1), 787-797; doi:10.3390/ijms15010787

Structural Characterization of a Water-Soluble Polysaccharide from the Fruiting Bodies of Agaricus bisporus

1
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China
2
Yuhang District Agricultural Monitoring Centre of Hangzhou, Hangzhou 311199, China
*
Author to whom correspondence should be addressed.
Received: 22 November 2013 / Revised: 12 December 2013 / Accepted: 17 December 2013 / Published: 8 January 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

An edible fungal polysaccharide termed as ABP was obtained by extraction with hot water, and followed successive chromatographic purification using DEAE-Sepharose Fast Flow column and Sephacryl S-300 High-Resolution column. A symmetrical peak was obtained on high-performance size-exclusion chromatography with an average molecular weight of 5.17 × 104 Da, which was named ABP, and its main components were D-glucose and D-mannose. Based on the study of methylation analysis, along with FT-IR, GC, GC-MS, 1D 1H and 13C NMR and 2D NMR (H-HCOSY, TOCSY, HMQC, and NOESY), its chemical structure was featured with a repeating unit (1→6) linking β-D-Glcp as the main backbone with (1→4)-linked α-D-Manp units. The structure of the mainly repeating units of ABP was established as: [PLEASE CHECK FORMULA IN THE PDF]
Keywords: Agaricus bisporus; polysaccharide; structural characterization; NMR spectrum Agaricus bisporus; polysaccharide; structural characterization; NMR spectrum
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

He, J.; Zhang, A.; Ru, Q.; Dong, D.; Sun, P. Structural Characterization of a Water-Soluble Polysaccharide from the Fruiting Bodies of Agaricus bisporus. Int. J. Mol. Sci. 2014, 15, 787-797.

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