Next Article in Journal
Towards Automated Binding Affinity Prediction Using an Iterative Linear Interaction Energy Approach
Previous Article in Journal
Dual Drug Release Electrospun Core-Shell Nanofibers with Tunable Dose in the Second Phase
Int. J. Mol. Sci. 2014, 15(1), 787-797; doi:10.3390/ijms15010787
Article

Structural Characterization of a Water-Soluble Polysaccharide from the Fruiting Bodies of Agaricus bisporus

1
, 1
, 2
, 2
 and 1,*
1 College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China 2 Yuhang District Agricultural Monitoring Centre of Hangzhou, Hangzhou 311199, China
* Author to whom correspondence should be addressed.
Received: 22 November 2013 / Revised: 12 December 2013 / Accepted: 17 December 2013 / Published: 8 January 2014
(This article belongs to the Section Bioactives and Nutraceuticals)
View Full-Text   |   Download PDF [360 KB, uploaded 19 June 2014]   |   Browse Figures

Abstract

An edible fungal polysaccharide termed as ABP was obtained by extraction with hot water, and followed successive chromatographic purification using DEAE-Sepharose Fast Flow column and Sephacryl S-300 High-Resolution column. A symmetrical peak was obtained on high-performance size-exclusion chromatography with an average molecular weight of 5.17 × 104 Da, which was named ABP, and its main components were D-glucose and D-mannose. Based on the study of methylation analysis, along with FT-IR, GC, GC-MS, 1D 1H and 13C NMR and 2D NMR (H-HCOSY, TOCSY, HMQC, and NOESY), its chemical structure was featured with a repeating unit (1→6) linking β-D-Glcp as the main backbone with (1→4)-linked α-D-Manp units. The structure of the mainly repeating units of ABP was established as: [PLEASE CHECK FORMULA IN THE PDF]
Keywords: Agaricus bisporus; polysaccharide; structural characterization; NMR spectrum Agaricus bisporus; polysaccharide; structural characterization; NMR spectrum
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Export to BibTeX |
EndNote


MDPI and ACS Style

He, J.; Zhang, A.; Ru, Q.; Dong, D.; Sun, P. Structural Characterization of a Water-Soluble Polysaccharide from the Fruiting Bodies of Agaricus bisporus. Int. J. Mol. Sci. 2014, 15, 787-797.

View more citation formats

Related Articles

Article Metrics

Comments

Citing Articles

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert