Int. J. Mol. Sci. 2014, 15(1), 44-57; doi:10.3390/ijms15010044
Article

Efficacy and Feasibility of the Epithelial Cell Adhesion Molecule (EpCAM) Immunomagnetic Cell Sorter for Studies of DNA Methylation in Colorectal Cancer

Received: 7 November 2013; in revised form: 10 December 2013 / Accepted: 13 December 2013 / Published: 20 December 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The aim of this work was to assess the impact on measurements of methylation of a panel of four cancer gene promoters of purifying tumor cells from colorectal tissue samples using the epithelial cell adhesion molecule (EpCAM)-immunomagnetic cell enrichment approach. We observed that, on average, methylation levels were higher in enriched cell fractions than in the whole tissue, but the difference was significant only for one out of four studied genes. In addition, there were strong correlations between methylation values for individual samples of whole tissue and the corresponding enriched cell fractions. Therefore, assays on whole tissue are likely to provide reliable estimates of tumor-specific methylation of cancer genes. However, tumor cell tissue separation using immunomagnetic beads could, in some cases, give a more accurate value of gene promoter methylation than the analysis of the whole cancer tissue, although relatively expensive and time-consuming. The efficacy and feasibility of the immunomagnetic cell sorting for methylation studies are discussed.
Keywords: DNA methylation; epigenetics; epithelial cell adhesion molecule (EpCAM); immunomagnetic enrichment; colon cancer; pyrosequencing
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MDPI and ACS Style

Failli, A.; Legitimo, A.; Migheli, F.; Coppedè, F.; Mathers, J.C.; Spisni, R.; Miccoli, P.; Migliore, L.; Consolini, R. Efficacy and Feasibility of the Epithelial Cell Adhesion Molecule (EpCAM) Immunomagnetic Cell Sorter for Studies of DNA Methylation in Colorectal Cancer. Int. J. Mol. Sci. 2014, 15, 44-57.

AMA Style

Failli A, Legitimo A, Migheli F, Coppedè F, Mathers JC, Spisni R, Miccoli P, Migliore L, Consolini R. Efficacy and Feasibility of the Epithelial Cell Adhesion Molecule (EpCAM) Immunomagnetic Cell Sorter for Studies of DNA Methylation in Colorectal Cancer. International Journal of Molecular Sciences. 2014; 15(1):44-57.

Chicago/Turabian Style

Failli, Alessandra; Legitimo, Annalisa; Migheli, Francesca; Coppedè, Fabio; Mathers, John C.; Spisni, Roberto; Miccoli, Paolo; Migliore, Lucia; Consolini, Rita. 2014. "Efficacy and Feasibility of the Epithelial Cell Adhesion Molecule (EpCAM) Immunomagnetic Cell Sorter for Studies of DNA Methylation in Colorectal Cancer." Int. J. Mol. Sci. 15, no. 1: 44-57.

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