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Phenological, Nutritional and Molecular Diversity Assessment among 35 Introduced Lentil (Lens culinaris Medik.) Genotypes Grown in Saudi Arabia
Legume Research Group, Plant production Department, Faculty of Food and Agricultural Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia
* Author to whom correspondence should be addressed.
Received: 17 September 2013; in revised form: 27 November 2013 / Accepted: 16 December 2013 / Published: 27 December 2013
Abstract: Morphological, nutritional and molecular analyses were carried out to assess genetic diversity among 35 introduced lentil genotypes (Lens culinaris Medik.). The genotypes exhibited significant differences for their field parameters and some of them showed noticeable superiority. The nutritional and proximate analysis showed that some genotypes were excellent sources of proteins, essential amino acids, minerals, anti-oxidants, total phenolic contents (TPC) and total flavonoid contents (TFC) and hence, highlights lentil nutritional and medicinal potential. Sequence-related amplified polymorphism (SRAP) and amplified fragments length polymorphism (AFLP) markers were used to estimate the genetic variability at the molecular level. The existence of a considerable amount of genetic diversity among the tested lentil genotypes was also proven at the molecular level. A total of 2894 polymorphic SRAP and 1625 AFLP loci were successfully amplified using six SRAP and four AFLP primer pair combinations. Polymorphism information content (PIC) values for SRAP and AFLP markers were higher than 0.8, indicating the power and higher resolution of those marker systems in detecting molecular diversity. UPGMA (unweighted pair group method with arithmetic average) cluster analysis based on molecular data revealed large number of sub clusters among genotypes, indicating high diversity levels. The data presented here showed that FLIP2009-64L and FLIP2009-69L could be used as a significant source of yield, total protein, essential amino acids, and antioxidant properties. The results suggest potential lentil cultivation in the central region of Saudi Arabia for its nutritional and medicinal properties, as well as sustainable soil fertility crop.
Keywords: lentil; nutritional value; molecular markers; genetic diversity
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MDPI and ACS Style
Alghamdi, S.S.; Khan, A.M.; Ammar, M.H.; El-Harty, E.H.; Migdadi, H.M.; El-Khalik, S.M.A.; Al-Shameri, A.M.; Javed, M.M.; Al-Faifi, S.A. Phenological, Nutritional and Molecular Diversity Assessment among 35 Introduced Lentil (Lens culinaris Medik.) Genotypes Grown in Saudi Arabia. Int. J. Mol. Sci. 2014, 15, 277-295.
Alghamdi SS, Khan AM, Ammar MH, El-Harty EH, Migdadi HM, El-Khalik SMA, Al-Shameri AM, Javed MM, Al-Faifi SA. Phenological, Nutritional and Molecular Diversity Assessment among 35 Introduced Lentil (Lens culinaris Medik.) Genotypes Grown in Saudi Arabia. International Journal of Molecular Sciences. 2014; 15(1):277-295.
Alghamdi, Salem S.; Khan, Altaf M.; Ammar, Megahed H.; El-Harty, Ehab H.; Migdadi, Hussein M.; El-Khalik, Samah M.A.; Al-Shameri, Aref M.; Javed, Muhammad M.; Al-Faifi, Sulieman A. 2014. "Phenological, Nutritional and Molecular Diversity Assessment among 35 Introduced Lentil (Lens culinaris Medik.) Genotypes Grown in Saudi Arabia." Int. J. Mol. Sci. 15, no. 1: 277-295.