DNA Repair Gene XRCC4 Codon 247 Polymorphism Modified Diffusely Infiltrating Astrocytoma Risk and Prognosis
Abstract
:1. Introduction
2. Results
2.1. Demographic and Clinic Characteristics of the Subjects
2.2. XRCC4 Codon 247 Polymorphism Increased DIA Risk
2.4. XRCC4 Codon 247 Polymorphism Modified DIA Prognosis
3. Discussion
4. Experimental Section
4.1. Study Population
4.2. DNA Extraction
4.3. Genotyping
4.4. DIA Patients Follow-up
4.5. Statistical Analysis
5. Conclusions
Acknowledgments
Conflicts of Interest
References
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Characteristics | Controls (n = 358) | Cases (n = 242) | χ2 | p | ||
---|---|---|---|---|---|---|
n | % | n | % | |||
Age (years) | 0.317 | 1.000 | ||||
≤35 | 19 | 5.3 | 13 | 5.4 | ||
36–40 | 23 | 6.4 | 16 | 6.6 | ||
41–45 | 33 | 9.2 | 22 | 9.1 | ||
46–50 | 76 | 21.2 | 53 | 21.9 | ||
51–55 | 102 | 28.5 | 68 | 28.1 | ||
56–60 | 45 | 12.6 | 30 | 12.4 | ||
61–65 | 34 | 9.5 | 24 | 9.9 | ||
≥66 | 26 | 7.3 | 15 | 6.2 | ||
Sex | 0.318 | 0.573 | ||||
Male | 230 | 64.2 | 150 | 62.0 | ||
Female | 128 | 35.8 | 92 | 38.0 | ||
Race | 0.026 | 0.872 | ||||
Han | 184 | 51.4 | 126 | 52.1 | ||
Minority | 174 | 48.6 | 116 | 47.9 |
XRCC4 | Controls | Cases | OR (95%CI) | p | |||
---|---|---|---|---|---|---|---|
n | % | n | % | ||||
Genotype | AA b | 298 | 83.2 | 168 | 69.4 | 1 | |
AS b | 39 | 10.9 | 40 | 16.5 | 1.82(1.13–2.94) a | 1.46 × 10−3 | |
SS b | 21 | 5.9 | 34 | 14.0 | 2.89(1.62–5.15) a | 3.22 × 10−4 | |
Allele | Ala c | 635 | 88.7 | 376 | 77.7 | 1 | |
Ser d | 81 | 11.3 | 108 | 22.3 | 2.25(1.64–3.09) | 4.43 × 10−7 |
Variable | Genotype | Control | Case | OR (95%CI) a | p | ||
---|---|---|---|---|---|---|---|
n | % | n | % | ||||
Race b | XRCC4 | ||||||
Han | AA | 152 | 82.6 | 87 | 69.0 | 1 | |
AS | 21 | 11.4 | 21 | 16.7 | 1.73(0.89–3.34) | 0.11 | |
SS | 11 | 5.8 | 18 | 14.2 | 3.07(1.35–7.00) | 7.68 × 10−3 | |
AS/SS | 32 | 17.2 | 39 | 30.9 | 2.16(1.25–3.71) | 5.65 × 10−3 | |
Zhuang | AA | 146 | 83.9 | 81 | 69.6 | 1 | |
AS | 18 | 10.2 | 19 | 16.4 | 1.87(0.93–3.76) | 0.08 | |
SS | 11 | 6.1 | 16 | 14.1 | 2.61(1.16–5.92) | 0.02 | |
AS/SS | 28 | 16.3 | 35 | 30.5 | 2.15(1.22–3.78) | 7.89 × 10−3 | |
Gender c | XRCC4 | ||||||
Male | AA | 193 | 83.9 | 104 | 69.3 | 1 | |
AS | 24 | 10.4 | 27 | 18.0 | 2.10(1.15–3.84) | 1.56 × 10−2 | |
SS | 13 | 5.8 | 19 | 12.7 | 2.81(1.33–5.96) | 6.95 × 10−3 | |
AS/SS | 37 | 16.2 | 46 | 30.7 | 2.35(1.43–3.86) | 7.82 × 10−4 | |
Female | AA | 105 | 82.0 | 64 | 69.3 | 1 | |
AS | 15 | 11.5 | 13 | 14.1 | 1.40(0.62–3.15) | 0.42 | |
SS | 8 | 6.2 | 15 | 16.5 | 2.86(1.14–7.19) | 2.50 × 10−2 | |
AS/SS | 23 | 17.8 | 28 | 30.6 | 1.92(1.01–3.64) | 4.60 × 10−2 | |
Age d | XRCC4 | ||||||
≤50 | AA | 128 | 84.8 | 75 | 72.1 | 1 | |
AS | 17 | 11.3 | 18 | 17.3 | 1.83(0.89–3.78) | 0.10 | |
SS | 6 | 3.8 | 11 | 10.6 | 3.19(1.13–9.02) | 2.85 × 10−2 | |
AS/SS | 151 | 99.9 | 29 | 27.9 | 2.19(1.18–4.06) | 1.34 × 10−2 | |
>50 | AA | 170 | 82.1 | 93 | 67.2 | 1 | |
AS | 22 | 10.5 | 22 | 15.9 | 1.86(0.98–3.56) | 0.06 | |
SS | 15 | 7.3 | 23 | 16.8 | 2.76(1.37–5.56) | 4.56 × 10−3 | |
AS/SS | 37 | 17.9 | 45 | 32.7 | 2.23(1.35–3.70) | 1.88 × 10−3 |
II | III | IV | ||||
---|---|---|---|---|---|---|
XRCC4 | n | % | n | % | n | % |
AA | 42 | 89.4 | 41 | 77.4 | 85 | 59.7 |
AS | 4 | 8.5 | 7 | 13.2 | 29 | 20.4 |
SS | 1 | 2.1 | 5 | 9.4 | 28 | 19.9 |
AS/SS | 5 | 10.6 | 12 | 22.6 | 57 | 40.3 |
Variable | HR(95%CI) | p |
---|---|---|
XRCC4 | ||
AA | 1 | |
AS | 2.26(1.57–3.24) | 9.75 × 10−6 |
SS | 5.36(3.51–8.17) | 6.42 × 10−15 |
Grade | ||
II | 1 | |
III | 4.27(2.53–7.20) | 5.32 × 10−8 |
IV | 9.27(5.54–15.51) | 2.31 × 10−17 |
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Lin, Z.-H.; Chen, J.-C.; Wang, Y.-S.; Huang, T.-J.; Wang, J.; Long, X.-D. DNA Repair Gene XRCC4 Codon 247 Polymorphism Modified Diffusely Infiltrating Astrocytoma Risk and Prognosis. Int. J. Mol. Sci. 2014, 15, 250-260. https://doi.org/10.3390/ijms15010250
Lin Z-H, Chen J-C, Wang Y-S, Huang T-J, Wang J, Long X-D. DNA Repair Gene XRCC4 Codon 247 Polymorphism Modified Diffusely Infiltrating Astrocytoma Risk and Prognosis. International Journal of Molecular Sciences. 2014; 15(1):250-260. https://doi.org/10.3390/ijms15010250
Chicago/Turabian StyleLin, Zhong-Hui, Jin-Chun Chen, Yun-Sun Wang, Teng-Jiao Huang, Jin Wang, and Xi-Dai Long. 2014. "DNA Repair Gene XRCC4 Codon 247 Polymorphism Modified Diffusely Infiltrating Astrocytoma Risk and Prognosis" International Journal of Molecular Sciences 15, no. 1: 250-260. https://doi.org/10.3390/ijms15010250